Pepsin A 抗体
Quick Overview for Pepsin A 抗体 (ABIN105444)
抗原
See all Pepsin A 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-1176-0100
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Supplier
- Rockland
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原理
- Pepsin Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach].
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产品特性
- Synonyms: goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody
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纯化方法
- Anti-PEPSIN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Anti-Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach.
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Pepsin A antibody
适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pepsin A (Internal Region) antibody适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Pepsin A antibody (Biotin)适用: Pig WB, IHC, ELISA 宿主: 山羊 Polyclonal Biotin
anti-Pepsin A antibody (HRP)适用: Pig WB, IHC, ELISA, DB 宿主: 山羊 Polyclonal HRP
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应用备注
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Immunohistochemistry Dilution: 1:2,000 - 1:10,000
Application Note: Anti-Pepsin antibody has been tested in ELISA, western blot and immunohistochemistry. This product detects a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:5,000 - 1:20,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:50:000 - 1:200,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Pepsin A
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别名
- PGA
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背景
- Background: Pepsin antibody detects Pepsin protein. Pepsin is an enzyme whose pepsinogen is released by the chief cells in the stomach and degrades food proteins into peptides. Pepsin belongs to the peptidase A1 family and is one of three principal proteolytic enzymes in the digestive system. Pepsin is most efficient in cleaving peptide bonds between hydrophobic and preferably aromatic amino acids such as phenylalanine, tryptophan, and tyrosine at positions P1 and P1'. Anti-Pepsin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.
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基因ID
- 396892
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UniProt
- P00791
抗原
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