ampC 抗体
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北京 101111
Quick Overview for ampC 抗体 (ABIN105438)
抗原
See all ampC 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4175-0100
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Supplier
- Rockland
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原理
- Penicillinase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Penicillinase [E.
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产品特性
- Synonyms: rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase
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纯化方法
- Anti-Penicillinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Penicillinase [E.cloacae]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-beta-Lactamase (ampC) antibody (Biotin)
ampC 适用: Enterobacter cloacae WB, IP, DB 宿主: 兔 Polyclonal Biotin
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应用备注
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Application Note: Penicillinase Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Penicillinase [E.cloacae] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:7,000 to 1:21,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- ampC (beta-Lactamase (ampC))
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别名
- ampC
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背景
- Background: Beta-lactamase or Penicillinase has catalytic activity of a beta-lactam + H2O = a substituted beta-amino acid.
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基因ID
- 9125732
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UniProt
- P05364
抗原
-
