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Ovalbumin 抗体 (HRP)

This anti-Ovalbumin antibody is a 兔 多克隆 antibody detecting Ovalbumin in WB, ELISA, IP 和 IHC. Suitable for 小鸡. This Primary Antibody has been cited in 1 publication.
Rockland
产品编号 ABIN105184
Supplier Product No.: 200-4333-0100
发货至: 中国

Quick Overview for Ovalbumin 抗体 (HRP) (ABIN105184)

抗原

See all Ovalbumin (OVA) 抗体
Ovalbumin (OVA)

适用

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小鸡

宿主

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克隆类型

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多克隆

标记

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This Ovalbumin antibody is conjugated to HRP

应用范围

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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
  • Supplier Product No.

    200-4333-0100

    Supplier

    Rockland

    原理

    Ovalbumin Antibody Peroxidase Conjugated

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White].

    产品特性

    Synonyms: rabbit anti-Ovalbumin Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Ovalbumin antibody, Egg albumin antibody, Plakalbumin, Allergen Gal d II, Allergen=Gal d 2, Hen Egg White

    纯化方法

    Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    免疫原

    Immunogen: Anti-Ovalbumin Antibody was produced by repeated immunizations with hen egg white Ovalbumin protein.

    Immunogen Type: Native Protein

    亚型

    IgG
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:2,500

    Application Note: Anti-Ovalbumin Antibody Peroxidase Conjugated has been tested by ELISA and western blot and is suitable for IHC and IP. Researchers should determine optimal titers for applications that are not stated below.

    Western Blot Dilution: 1:1,000 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度

    1.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

    储存液

    Gentamicin sulfate

    注意事项

    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    有效期

    12 months
  • Apperson, Vorachek, Dolan, Bobe, Pirelli, Hall: "Effects of feeding pregnant beef cows selenium-enriched alfalfa hay on passive transfer of ovalbumin in their newborn calves." in: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 50, pp. 640-645, (2019) (PubMed).

  • 抗原

    Ovalbumin (OVA)

    别名

    SERPINB14

    背景

    Background: Anti-Ovalbumin Antibody detects Ovalbumin. Ovalbumin is the main protein found in egg white, making up 60-65 % of the total protein. It is a non-inhibitory serpin. It is the storage protein of egg white. Anti-Ovalbumin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

    基因ID

    396058

    UniProt

    P01012
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