SLC44A2 抗体 (Internal Region)
Quick Overview for SLC44A2 抗体 (Internal Region) (ABIN1049339)
抗原
See all SLC44A2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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品牌
- IHC-plus™
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特异性
- Human SLC44A2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
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预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Gibbon, Monkey, Marmoset (94%) Orangutan, Galago, Dog (88%) Rat, Hamster, Elephant, Panda, Horse, Pig, Guinea pig (81%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic 16 amino acid peptide from internal region of human SLC44A2. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Gibbon, Monkey, Marmoset (94%), Orangutan, Galago, Dog (88%), Rat, Hamster, Elephant, Panda, Horse, Pig, Guinea pig (81%).
Type of Immunogen: Synthetic peptide
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anti-Solute Carrier Family 44, Member 2 (SLC44A2) (AA 123-231) antibody
SLC44A2 适用: 人 IHC, ELISA, WB, IHC (p) 宿主: 小鼠 Monoclonal 3D11 unconjugated
anti-Solute Carrier Family 44, Member 2 (SLC44A2) (AA 123-230) antibodySLC44A2 适用: 人 ELISA, WB, IHC (p) 宿主: 小鼠 Monoclonal 3D11 unconjugated
anti-Solute Carrier Family 44, Member 2 (SLC44A2) (AA 123-231) antibodySLC44A2 适用: 人 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3D11 unconjugated
anti-Solute Carrier Family 44, Member 2 (SLC44A2) (AA 123-230) antibodySLC44A2 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 1D5 unconjugated
anti-Solute Carrier Family 44, Member 2 (SLC44A2) antibodySLC44A2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. -
说明
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Target Species of Antibody: Human
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实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, less than 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
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有效期
- 12 months
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- SLC44A2 (Solute Carrier Family 44, Member 2 (SLC44A2))
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别名
- SLC44A2 / CTL2
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背景
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Name/Gene ID: SLC44A2
Subfamily: Choline transporter-like
Family: Transporter
Synonyms: SLC44A2, CTL2, PP1292 -
基因ID
- 57153
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途径
- SARS-CoV-2 Protein Interactome
抗原
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