电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

RORA 抗体 (Ligand Binding Domain)

RORA 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 猴, Pig, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1049294
发货至: 中国
  • 抗原 See all RORA 抗体
    RORA (RAR-Related Orphan Receptor A (RORA))
    抗原表位
    • 15
    • 9
    • 7
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ligand Binding Domain
    适用
    • 78
    • 42
    • 41
    • 12
    • 10
    • 9
    • 9
    • 8
    • 7
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 猴, Pig, Bat
    宿主
    • 73
    • 5
    克隆类型
    • 74
    • 4
    多克隆
    标记
    • 44
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This RORA antibody is un-conjugated
    应用范围
    • 69
    • 28
    • 14
    • 10
    • 10
    • 3
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human ROR Alpha. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    预测反应
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%) Turkey, Chicken, Xenopus (95%) Stickleback, Pufferfish, Zebrafish (90%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic 20 amino acid peptide from ligand-binding domain of human ROR Alpha. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Platypus (100%), Turkey, Chicken, Xenopus (95%), Stickleback, Pufferfish, Zebrafish (90%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product RORA Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (3 μg/mL)
    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, less than 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    RORA (RAR-Related Orphan Receptor A (RORA))
    别名
    RORA / ROR alpha (RORA 产品)
    别名
    ror1 antibody, ror2 antibody, ror3 antibody, rzra antibody, nr1f1 antibody, MGC146531 antibody, NR1F1 antibody, ROR1 antibody, ROR2 antibody, ROR3 antibody, RZR-ALPHA antibody, RZRA antibody, RORalpha-B antibody, gb:dq017624 antibody, rora2 antibody, 9530021D13Rik antibody, Nr1f1 antibody, nmf267 antibody, sg antibody, staggerer antibody, tmgc26 antibody, RORalpha1 antibody, RAR related orphan receptor A antibody, RAR-related orphan receptor A antibody, RAR-related orphan receptor A, paralog a antibody, RAR-related orphan receptor alpha antibody, RORA antibody, rora antibody, Rora antibody, roraa antibody
    背景
    Name/Gene ID: RORA
    Subfamily: NR1 Thyroid hormone-like
    Family: NHR

    Synonyms: RORA, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, Roralpha, Transcription factor RZR-alpha, RAR-related orphan receptor A, ROR-alpha, ROR3, RZRA, NR1F1, ROR Alpha, RZR-ALPHA
    基因ID
    6095
    途径
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
You are here:
客服