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MRGPRD 抗体 (Extracellular Domain)

This anti-MRGPRD antibody is a 兔 多克隆 antibody detecting MRGPRD in IHC 和 IHC (p). Suitable for 人.
产品编号 ABIN1049068
发货至: 中国

Quick Overview for MRGPRD 抗体 (Extracellular Domain) (ABIN1049068)

抗原

See all MRGPRD 抗体
MRGPRD (MAS-Related GPR, Member D (MRGPRD))

适用

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宿主

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克隆类型

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多克隆

标记

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This MRGPRD antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    Extracellular Domain

    品牌

    IHC-plus™

    特异性

    Human MRGPRD. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

    预测反应

    Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Gibbon, Monkey (94%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic 18 amino acid peptide from 2nd extracellular domain of human MRGPRD. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Gibbon, Monkey (94%).

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: IHC, IHC-P (9.3 μg/mL)

    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, less than 0.1 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    MRGPRD (MAS-Related GPR, Member D (MRGPRD))

    别名

    MRGD / MRGPRD

    背景

    Name/Gene ID: MRGPRD
    Subfamily: Orphan-A
    Family: GPCR

    Synonyms: MRGPRD, Beta-alanine receptor, MRGD, MAS-related GPR, member D, TGR7

    基因ID

    116512
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