MMP13 抗体 (Internal Region)
Quick Overview for MMP13 抗体 (Internal Region) (ABIN1049066)
抗原
See all MMP13 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Internal Region
-
品牌
- IHC-plus™
-
特异性
- Human MMP13. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
-
预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Horse, Rabbit, Pig (100%) Mouse, Rat, Sheep, Dog, Hamster (94%) Bovine, Opossum, Turkey (89%) Chicken (83%).
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic 18 amino acid peptide from internal region of human MMP13. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Elephant, Panda, Horse, Rabbit, Pig (100%), Mouse, Rat, Sheep, Dog, Hamster (94%), Bovine, Opossum, Turkey (89%), Chicken (83%).
Type of Immunogen: Synthetic peptide
-
-
anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 201-300) antibody
MMP13 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 103-290) antibodyMMP13 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) antibodyMMP13 适用: 人 WB, IHC, ICC, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 290-468) antibodyMMP13 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 362-471) antibodyMMP13 适用: 人 WB, ELISA, IP, RNAi 宿主: 小鼠 Monoclonal 3B11 unconjugated
anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 99-335) antibodyMMP13 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 70-245) antibody (APC)MMP13 适用: 兔 WB 宿主: 豚鼠 Polyclonal APC
anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 104-471) antibodyMMP13 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (AA 123-270) antibodyMMP13 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) (N-Term) antibodyMMP13 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Approved: IHC, IHC-P (15 - 25 μg/mL)
-
说明
-
Target Species of Antibody: Human
-
实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, less than 0.1 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
-
有效期
- 12 months
-
-
- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
-
别名
- MMP13
-
背景
-
Name/Gene ID: MMP13
Subfamily: Metallopeptidase M10A
Family: Protease
Synonyms: MMP13, Collagenase 3, Matrix metalloproteinase-13, Interstitial collagenase, MANDP1, MMP-13, CLG3 -
基因ID
- 4322
抗原
-
