LPHN2 抗体 (N-Term)
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- 抗原 See all LPHN2 抗体
- LPHN2 (Latrophilin 2 (LPHN2))
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抗原表位
- N-Term
- 适用
- 人, 大鼠, 小鼠, Cow, 犬, 马, Pig, 兔, Bat
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LPHN2 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human LPHN2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Platypus (100%) Marmoset, Chicken, Xenopus, Pufferfish (94%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 16 amino acid peptide from N-terminal extracellular domain of human LPHN2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bat, Bovine, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Platypus (100%), Marmoset, Chicken, Xenopus, Pufferfish (94%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product LPHN2 Primary Antibody
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anti-Latrophilin 2 (LPHN2) (AA 400-480) antibody
LPHN2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Latrophilin 2 (LPHN2) (C-Term) antibodyLPHN2 适用: 人, Gorilla IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Latrophilin 2 (LPHN2) (N-Term) antibodyLPHN2 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Latrophilin 2 (LPHN2) (N-Term) antibodyLPHN2 适用: 人, 大鼠, 小鼠, Cow, 犬, 马, Pig, 兔, Gorilla IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Latrophilin 2 (LPHN2) (AA 139-398) antibodyLPHN2 适用: 大鼠 IHC, WB, ICC 宿主: 兔 Polyclonal unconjugated
anti-Latrophilin 2 (LPHN2) (Center) antibodyLPHN2 适用: 人, 大鼠, 小鼠, Cow IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Latrophilin 2 (LPHN2) (AA 551-600) antibodyLPHN2 适用: 人, 大鼠, 小鼠 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC, IHC-P (7 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- LPHN2 (Latrophilin 2 (LPHN2))
- 别名
- ADGRL2 / LPHN2 (LPHN2 产品)
- 别名
- LPHN2 antibody, CIRL2 antibody, CL2 antibody, LEC1 antibody, LPHH1 antibody, AI450192 antibody, Gm619 antibody, Lec1 antibody, Lphh1 antibody, mKIAA0786 antibody, Cirl-2 antibody, Cirl2 antibody, Cl2ac antibody, LEC2 antibody, adhesion G protein-coupled receptor L2 antibody, ADGRL2 antibody, adgrl2 antibody, LOC100538279 antibody, Adgrl2 antibody
- 背景
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Name/Gene ID: ADGRL2
Subfamily: Orphan-B
Family: GPCR
Synonyms: ADGRL2, Cl2aa, CIRL2, CL2, Latrophilin 1, Latrophilin-2, Lectomedin-1, Latrophilin 2, Latrophilin homolog 2 (cow), KIAA0786, Beta-latrotoxin receptor, CIRL-2, Latrophilin homolog 1, Latrotoxin receptor 2, Latrotoxin receptor homolog 2, LEC1, LPHH1, LPHN2 - 基因ID
- 23266
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