LPAR4 抗体 (Cytoplasmic Domain)
Quick Overview for LPAR4 抗体 (Cytoplasmic Domain) (ABIN1049015)
抗原
See all LPAR4 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Cytoplasmic Domain
-
品牌
- IHC-plus™
-
特异性
- Human LPAR4 / GPR23. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
-
预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Rabbit (100%) Bovine, Pig, Opossum, Turkey, Chicken (94%) Xenopus (88%).
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic 17 amino acid peptide from 3rd cytoplasmic domain of human LPAR4 / GPR23. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Horse, Rabbit (100%), Bovine, Pig, Opossum, Turkey, Chicken (94%), Xenopus (88%).
Type of Immunogen: Synthetic peptide
-
-
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (AA 175-270) antibody
LPAR4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (AA 1-43) antibodyLPAR4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (N-Term) antibodyLPAR4 适用: 人, 小鼠, 大鼠, Bat, 猴, Hamster IHC, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (C-Term) antibodyLPAR4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (C-Term) antibodyLPAR4 适用: 人, 犬, Cow, Pig, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (AA 3-16), (Extracellular) antibodyLPAR4 适用: 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Approved: IHC, IHC-P (4 μg/mL)
-
说明
-
Target Species of Antibody: Human
-
实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, less than 0.1 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
-
有效期
- 12 months
-
-
- LPAR4 (Lysophosphatidic Acid Receptor 4 (LPAR4))
-
别名
- LPAR4 / GPR23
-
背景
-
Name/Gene ID: LPAR4
Subfamily: Lysophospholipid/Lysosphingolipid
Family: GPCR
Synonyms: LPAR4, G-protein coupled receptor 23, LPA-4, p2RY9, p2Y5-LIKE, p2Y purinoceptor 9, p2Y5-like receptor, p2Y9, G protein-coupled receptor 23, GPR23, LPA receptor 4, LPA4, Purinergic receptor 9 -
基因ID
- 2846
抗原
-
