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KCNA3 抗体 (C-Term)

KCNA3 适用: 人, 小鼠, 大鼠, Pig, 兔, 犬, Hamster, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN1048981
发货至: 中国
  • 抗原 See all KCNA3 抗体
    KCNA3 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 3 (KCNA3))
    抗原表位
    • 16
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    C-Term
    适用
    • 76
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    • 3
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    • 1
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    人, 小鼠, 大鼠, Pig, 兔, 犬, Hamster, 猴
    宿主
    • 74
    • 3
    • 1
    克隆类型
    • 75
    • 3
    多克隆
    标记
    • 31
    • 5
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    This KCNA3 antibody is un-conjugated
    应用范围
    • 51
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    • 15
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    • 7
    • 5
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    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    品牌
    IHC-plus™
    特异性
    Human KCNA3 / Kv1.3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    预测反应
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit, Pig (100%) Bovine, Opossum (94%) Lizard (88%) Turkey, Chicken (81%).
    纯化方法
    Immunoaffinity purified
    免疫原
    Synthetic 16 amino acid peptide from C-terminus of human KCNA3 / Kv1.3. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Rabbit, Pig (100%), Bovine, Opossum (94%), Lizard (88%), Turkey, Chicken (81%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product KCNA3 Primary Antibody
  • 应用备注
    Approved: IHC, IHC-P (5 μg/mL)

    Usage: Antigen retrieval requires Proteinase K treatment.
    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, less than 0.1 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    KCNA3 (Potassium Voltage-Gated Channel, Shaker-Related Subfamily, Member 3 (KCNA3))
    别名
    KCNA3 / Kv1.3 (KCNA3 产品)
    别名
    Kv1.3-glyb antibody, kv1.3 antibody, Kv1.3B antibody, kcna3b-a antibody, HGK5 antibody, HLK3 antibody, HPCN3 antibody, HUKIII antibody, KV1.3 antibody, MK3 antibody, PCN3 antibody, Kca1-3 antibody, Kv1.3 antibody, Mk-3 antibody, cKv1.1 antibody, potassium voltage-gated channel subfamily A member 3 antibody, potassium channel, voltage gated shaker related subfamily A, member 3 S homeolog antibody, potassium voltage-gated channel, shaker-related subfamily, member 3 antibody, KCNA3 antibody, kcna3.S antibody, Kcna3 antibody
    背景
    Name/Gene ID: KCNA3
    Subfamily: Potassium channel - Kv1 Shaker
    Family: Ion Channel

    Synonyms: KCNA3, HGK5, HPCN3, HUKIII, KV1.3, MK3, HLK3, HUK3, Potassium channel 3, Type n potassium channel, PCN3
    基因ID
    3738
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