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GPER 抗体 (Cytoplasmic Domain)

This anti-GPER antibody is a 兔 多克隆 antibody detecting GPER in WB, IHC (p), IHC 和 ICC. Suitable for 人. This Primary Antibody has been cited in 2+ publications.
产品编号 ABIN1048791
发货至: 中国

Quick Overview for GPER 抗体 (Cytoplasmic Domain) (ABIN1048791)

抗原

See all GPER 抗体
GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

适用

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宿主

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克隆类型

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多克隆

标记

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This GPER antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
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    Cytoplasmic Domain

    品牌

    IHC-plus™

    特异性

    Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

    预测反应

    Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Marmoset (94%) Monkey, Panda (88%).

    纯化方法

    Immunoaffinity purified

    免疫原

    Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Marmoset (94%), Monkey, Panda (88%).

    Type of Immunogen: Synthetic peptide
  • 应用备注

    Approved: ICC, IHC, IHC-P (15 - 20 μg/mL), WB

    Not recommended for: IF

    说明

    Target Species of Antibody: Human

    实验流程

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, less than 0.1 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

    有效期

    12 months
  • Vivacqua, Bonofiglio, Recchia, Musti, Picard, Andò, Maggiolini: "The G protein-coupled receptor GPR30 mediates the proliferative effects induced by 17beta-estradiol and hydroxytamoxifen in endometrial cancer cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 20, Issue 3, pp. 631-46, (2006) (PubMed).

    Thomas, Pang, Filardo, Dong: "Identity of an estrogen membrane receptor coupled to a G protein in human breast cancer cells." in: Endocrinology, Vol. 146, Issue 2, pp. 624-32, (2005) (PubMed).

  • 抗原

    GPER (G Protein-Coupled Estrogen Receptor 1 (GPER))

    别名

    GPER1 / GPR30

    背景

    Name/Gene ID: GPER1
    Subfamily: Transmembrane Estrogen Receptor
    Family: GPCR

    Synonyms: GPER1, CMKRL2, CEPR, FEG-1, G-protein coupled receptor 30, G protein-coupled receptor 30, GPCR-Br, GPER, Heptahelix receptor, LERGU, LyGPR, MER, GPR30, IL8-related receptor DRY12, LERGU2, Chemokine receptor-like 2, DRY12, Membrane estrogen receptor

    基因ID

    2852

    途径

    EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
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