GPR137B 抗体 (Cytoplasmic Domain)
-
- 抗原 See all GPR137B products
- GPR137B (G Protein-Coupled Receptor 137B (GPR137B))
-
抗原表位
- Cytoplasmic Domain
- 适用
- 人, 小鼠, 大鼠, 马, 猴, Pig, 兔, Cow, 犬, Hamster, 小鸡
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This GPR137B antibody is un-conjugated
-
应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 品牌
- IHC-plus™
- 特异性
- Human GPR137B / TM7SF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard (100%) Goat, Panda, Xenopus, Zebrafish (94%) Platypus, Stickleback (88%) Pufferfish (81%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 16 amino acid peptide from 2nd cytoplasmic domain of human GPR137B / TM7SF1. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard (100%), Goat, Panda, Xenopus, Zebrafish (94%), Platypus, Stickleback (88%), Pufferfish (81%).
Type of Immunogen: Synthetic peptide
-
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibody
GPR137B 适用: 人, 小鼠, 大鼠, 马, 猴, Pig, 兔, Cow, 犬, Hamster IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibodyGPR137B 适用: 人, 小鼠, 大鼠, 马, 猴, Pig, 兔 IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibodyGPR137B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibodyGPR137B 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibodyGPR137B 适用: 人 IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 137B (GPR137B) (C-Term) antibodyGPR137B 适用: 人 IHC (p), IF 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: ICC (6 - 12 μg/mL), IHC, IHC-P (6 - 12 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- GPR137B (G Protein-Coupled Receptor 137B (GPR137B))
- 别名
- GPR137B (GPR137B 产品)
- 别名
- TM7SF1 antibody, AI788917 antibody, AU016435 antibody, AW546472 antibody, C730041E01 antibody, C80550 antibody, C80741 antibody, D13Abb1e antibody, Tm7sf1 antibody, GPR137B antibody, gpr137ba antibody, tm7sf1a antibody, wu:fi39g02 antibody, zgc:101671 antibody, G protein-coupled receptor 137B antibody, G protein-coupled receptor 137B L homeolog antibody, G protein-coupled receptor 137c antibody, GPR137B antibody, gpr137b.L antibody, Gpr137b antibody, gpr137b antibody, gpr137c antibody
- 背景
-
Name/Gene ID: GPR137B
Subfamily: Orphan-U
Family: GPCR
Synonyms: GPR137B, TM7SF1 - 基因ID
- 7107
-
