G Protein-Coupled Receptor 133 抗体 (Cytoplasmic Domain)
Quick Overview for G Protein-Coupled Receptor 133 抗体 (Cytoplasmic Domain) (ABIN1048714)
抗原
See all G Protein-Coupled Receptor 133 (GPR133) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Cytoplasmic Domain
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品牌
- IHC-plus™
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特异性
- Human GPR133. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
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预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog, Panda, Rabbit, Pig (100%) Gibbon, Bovine, Elephant, Horse (95%) Mouse, Hamster (84%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human GPR133. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog, Panda, Rabbit, Pig (100%), Gibbon, Bovine, Elephant, Horse (95%), Mouse, Hamster (84%).
Type of Immunogen: Synthetic peptide
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anti-G Protein-Coupled Receptor 133 (GPR133) antibody
GPR133 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 133 (GPR133) (C-Term) antibodyGPR133 适用: 人 IHC (p), IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 133 (GPR133) (Internal Region) antibodyGPR133 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 133 (GPR133) (AA 805-874) antibodyGPR133 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 133 (GPR133) (AA 461-510) antibodyGPR133 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Approved: IHC, IHC-P (10 - 20 μg/mL)
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说明
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Target Species of Antibody: Human
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实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, less than 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
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有效期
- 12 months
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- G Protein-Coupled Receptor 133 (GPR133)
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别名
- ADGRD1 / GPR133
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背景
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Name/Gene ID: ADGRD1
Subfamily: Orphan-U
Family: GPCR
Synonyms: ADGRD1, G protein-coupled receptor 133, GPR133, PGR25 -
基因ID
- 283383
抗原
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