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- 抗原 See all ELOVL5 抗体
- ELOVL5 (ELOVL Fatty Acid Elongase 5 (ELOVL5))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ELOVL5 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human ELOVL5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Marmoset, Panda (93%) Goat, Dog, Bat, Bovine, Rabbit, Opossum (87%) Elephant, Horse, Turkey, Chicken, Lizard (80%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 15 amino acid peptide from C-Terminus of human ELOVL5. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset, Panda (93%), Goat, Dog, Bat, Bovine, Rabbit, Opossum (87%), Elephant, Horse, Turkey, Chicken, Lizard (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product ELOVL5 Primary Antibody
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anti-ELOVL Fatty Acid Elongase 5 (ELOVL5) (N-Term) antibody
ELOVL5 适用: 人, 大鼠, 小鼠, 兔, 豚鼠, Cow, 犬, 山羊, 马, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-ELOVL Fatty Acid Elongase 5 (ELOVL5) (Internal Region) antibodyELOVL5 适用: 人, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ELOVL Fatty Acid Elongase 5 (ELOVL5) antibodyELOVL5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ELOVL Fatty Acid Elongase 5 (ELOVL5) (C-Term) antibodyELOVL5 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ELOVL Fatty Acid Elongase 5 (ELOVL5) (C-Term) antibodyELOVL5 适用: 人, Gorilla, Orang-Utan IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ELOVL Fatty Acid Elongase 5 (ELOVL5) (AA 211-299) antibodyELOVL5 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-ELOVL Fatty Acid Elongase 5 (ELOVL5) (AA 247-299) antibodyELOVL5 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ELOVL Fatty Acid Elongase 5 (ELOVL5) (N-Term) antibodyELOVL5 适用: 人, 小鼠, 猴, 兔, 豚鼠, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-ELOVL Fatty Acid Elongase 5 (ELOVL5) (AA 247-326) antibodyELOVL5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-ELOVL Fatty Acid Elongase 5 (ELOVL5) (AA 271-299), (C-Term) antibodyELOVL5 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB23876 unconjugated
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- 应用备注
- Approved: IHC, IHC-P (15 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- ELOVL5 (ELOVL Fatty Acid Elongase 5 (ELOVL5))
- 别名
- HELO1 / ELOVL5 (ELOVL5 产品)
- 别名
- zgc:63549 antibody, elovl2 antibody, 1110059L23Rik antibody, AI747313 antibody, AU043003 antibody, HELO1 antibody, dJ483K16.1 antibody, rELO1 antibody, ELOVL fatty acid elongase 5 antibody, ELOVL family member 5, elongation of long chain fatty acids (yeast) antibody, ELOVL fatty acid elongase 5 S homeolog antibody, elovl5 antibody, ELOVL5 antibody, Elovl5 antibody, elovl5.S antibody
- 背景
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Name/Gene ID: ELOVL5
Synonyms: ELOVL5, ELOVL FA elongase 5, ELOVL fatty acid elongase 5, Fatty acid elongase 1, RP3-483K16.1, DJ483K16.1, HELO1 - 基因ID
- 60481
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