CDC7 抗体 (Kinase Domain)
-
- 抗原 See all CDC7 抗体
- CDC7 (Cell Division Cycle 7 (CDC7))
-
抗原表位
- Kinase Domain
-
适用
- 人, 犬, 马, Cow, 兔, Bat
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This CDC7 antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CDC7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%) Monkey, Mouse, Rat, Hamster, Platypus (95%) Turkey, Chicken, Lizard, Xenopus (89%) Stickleback, Pufferfish, Zebrafish (84%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
-
Synthetic 19 amino acid peptide from kinase domain of human CDC7. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum (100%), Monkey, Mouse, Rat, Hamster, Platypus (95%), Turkey, Chicken, Lizard, Xenopus (89%), Stickleback, Pufferfish, Zebrafish (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CDC7 Primary Antibody
-
anti-Cell Division Cycle 7 (CDC7) (AA 275-574) antibody
CDC7 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 7 (CDC7) (N-Term) antibodyCDC7 适用: 人, 小鼠 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 7 (CDC7) (AA 1-30), (N-Term) antibodyCDC7 适用: 人 WB 宿主: 兔 Polyclonal RB3541 unconjugated
anti-Cell Division Cycle 7 (CDC7) antibodyCDC7 适用: 人 WB, IHC (p), IP 宿主: 小鼠 Monoclonal DCS-342 unconjugated
anti-Cell Division Cycle 7 (CDC7) (AA 151-250) antibodyCDC7 适用: 人, 大鼠 ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 7 (CDC7) (AA 151-250) antibody (FITC)CDC7 适用: 人, 大鼠 FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal FITC
anti-Cell Division Cycle 7 (CDC7) antibodyCDC7 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 7 (CDC7) antibodyCDC7 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Approved: IHC, IHC-P (6.5 μg/mL)
- 说明
-
Target Species of Antibody: Human
- 实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- CDC7 (Cell Division Cycle 7 (CDC7))
- 别名
- CDC7 (CDC7 产品)
- 别名
- CDC7L1 antibody, HsCDC7 antibody, Hsk1 antibody, huCDC7 antibody, AI597260 antibody, Cdc7l1 antibody, muCdc7 antibody, cell division cycle 7 antibody, cell division cycle 7-related protein kinase antibody, cell division cycle 7 (S. cerevisiae) antibody, CDC7 antibody, Tsp_08585 antibody, Cdc7 antibody
- 背景
-
Name/Gene ID: CDC7
Subfamily: CDC7
Family: Protein Kinase
Synonyms: CDC7, Cdc7 Kinase, CDC7-like 1, CDC7L1, HsCDC7, HuCDC7, CDC7-related kinase, Hsk1, MuCdc7 - 基因ID
- 8317
- 途径
- Mitotic G1-G1/S Phases, DNA Replication
-
