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- 抗原 See all CCR9 抗体
- CCR9 (Chemokine (C-C Motif) Receptor 9 (CCR9))
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抗原表位
- Extracellular Domain
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CCR9 antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human CCR9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey (95%) Horse, Pig (89%) Bovine (84%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 19 amino acid peptide from 2nd extracellular domain of human CCR9. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey (95%), Horse, Pig (89%), Bovine (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CCR9 Primary Antibody
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anti-Chemokine (C-C Motif) Receptor 9 (CCR9) antibody
CCR9 适用: 人 WB, ELISA, IF, IP 宿主: 兔 Monoclonal 4G2 unconjugated Recombinant Antibody
anti-Chemokine (C-C Motif) Receptor 9 (CCR9) (AA 3-22) antibodyCCR9 适用: 小鼠 FACS, IF, ICC, IHC (fro) 宿主: 大鼠 Monoclonal 7E7 unconjugated
anti-Chemokine (C-C Motif) Receptor 9 (CCR9) antibodyCCR9 适用: 人 FACS 宿主: 小鼠 Monoclonal C9Mab-1 unconjugated
anti-Chemokine (C-C Motif) Receptor 9 (CCR9) antibodyCCR9 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 9 (CCR9) (Cytoplasmic Domain) antibodyCCR9 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 9 (CCR9) (C-Term) antibodyCCR9 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 9 (CCR9) (AA 65-160) antibodyCCR9 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 9 (CCR9) (N-Term) antibodyCCR9 适用: 小鼠 FACS, Neut 宿主: 兔 Chimeric 9B1 unconjugated Recombinant Antibody
anti-Chemokine (C-C Motif) Receptor 9 (CCR9) (AA 1-369) antibodyCCR9 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 9 (CCR9) (AA 1-100) antibodyCCR9 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Approved: IHC-P (2 - 3 μg/mL)
- 说明
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This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
- 有效期
- 12 months
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- 抗原
- CCR9 (Chemokine (C-C Motif) Receptor 9 (CCR9))
- 别名
- CCR9 (CCR9 产品)
- 别名
- CC-CKR-9 antibody, CDw199 antibody, GPR-9-6 antibody, GPR28 antibody, A130091K22Rik antibody, Cmkbr10 antibody, C-C motif chemokine receptor 9 antibody, chemokine (C-C motif) receptor 9 antibody, CCR9 antibody, Ccr9 antibody
- 背景
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Name/Gene ID: CCR9
Subfamily: Chemokine
Family: GPCR
Synonyms: CCR9, C-C CKR-9, CC-CKR-9, CCR-9, CDw199 antigen, C-C chemokine receptor 9, C-C chemokine receptor type 9, Chemokine c-c motif receptor 9, CDw199, G protein-coupled receptor 28, GPR-9-6, G-protein coupled receptor 28, GPR28 - 基因ID
- 10803
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