C14orf1 抗体 (Internal Region)
Quick Overview for C14orf1 抗体 (Internal Region) (ABIN1048360)
抗原
适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Internal Region
-
品牌
- IHC-plus™
-
特异性
- Human C14orf1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except C14orf1 (100 % ).
-
预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Platypus (100%) Horse, Guinea pig (92%) Salmon, Smelt, Stickleback, Medaka, Pufferfish (83%).
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic 12 amino acid peptide from internal region of human C14orf1. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig, Opossum, Platypus (100%), Horse, Guinea pig (92%), Salmon, Smelt, Stickleback, Medaka, Pufferfish (83%).
Type of Immunogen: Synthetic peptide
-
-
anti-Chromosome 14 Open Reading Frame 1 (C14orf1) (Internal Region) antibody
C14orf1 适用: 人, 小鼠, 猴, 兔, 豚鼠, 犬, Hamster, 大鼠, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chromosome 14 Open Reading Frame 1 (C14orf1) (C-Term) antibodyC14orf1 适用: 人, 猴, 兔, Bat, 豚鼠 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Approved: IHC, IHC-P (5 - 7.5 μg/mL)
Usage: Immunohistochemistry with formalin-fixed paraffin-embedded tissues requires pretreatment using HIER with citrate buffer. -
说明
-
Target Species of Antibody: Human
-
实验流程
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, less than 0.1 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
-
有效期
- 12 months
-
-
- C14orf1 (Chromosome 14 Open Reading Frame 1 (C14orf1))
-
别名
- C14orf1
-
背景
-
Name/Gene ID: C14orf1
Synonyms: C14orf1, ERG28, NET51 -
基因ID
- 11161
抗原
-
