5HT1B Receptor 抗体 (Internal Region)
Quick Overview for 5HT1B Receptor 抗体 (Internal Region) (ABIN1048254)
抗原
See all 5HT1B Receptor (HTR1B) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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品牌
- IHC-plus™
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特异性
- Human 5HT1B Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
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预测反应
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse, Rat, Bat, Horse, Pig (94%) Hamster, Panda, Bovine, Dog, Cat, Rabbit, Guinea pig (88%) Elephant, Opossum (82%).
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic 17 amino acid peptide from internal region of human 5HT1B Receptor. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse, Rat, Bat, Horse, Pig (94%), Hamster, Panda, Bovine, Dog, Cat, Rabbit, Guinea pig (88%), Elephant, Opossum (82%).
Type of Immunogen: Synthetic peptide
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anti-Serotonin Receptor 1B (HTR1B) (Internal Region) antibody
HTR1B 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1B (HTR1B) (AA 1-49) antibodyHTR1B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Serotonin Receptor 1B (HTR1B) (Cytoplasmic Domain) antibodyHTR1B 适用: 人, 大鼠, 小鼠, 犬, Bat, Cow, 豚鼠, Hamster, 兔, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1B (HTR1B) (C-Term) antibodyVerified HTR1B 适用: 人, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Serotonin Receptor 1B (HTR1B) (AA 33-49), (Extracellular) antibodyHTR1B 适用: 人 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1B (HTR1B) (AA 8-26) antibodyHTR1B 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1B (HTR1B) (N-Term) antibodyHTR1B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1B (HTR1B) (AA 261-310) antibodyHTR1B 适用: 人, 大鼠, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Serotonin Receptor 1B (HTR1B) (AA 201-250) antibodyHTR1B 适用: 人, 大鼠, 小鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: IHC, IHC-P (10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. -
说明
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Target Species of Antibody: Human
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实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, less than 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
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有效期
- 12 months
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- 5HT1B Receptor (HTR1B) (Serotonin Receptor 1B (HTR1B))
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别名
- HTR1B / 5-HT1B Receptor
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背景
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Name/Gene ID: HTR1B
Subfamily: Serotonin
Family: GPCR
Synonyms: HTR1B, 5-HT-1B, 5-HT-1D-beta, 5-HT1B, 5-HT1b receptor, 5-HT1DB, 5HT1B Receptor, HTR1DB, Serotonin 5-HT-1b receptor, Serotonin receptor 1B, Serotonin 1b receptor, HTR1D2, Serotonin 1D beta receptor -
基因ID
- 3351
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途径
- JAK/STAT Signaling, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Feeding Behaviour, S100 Proteins
抗原
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