NFYB 抗体 (N-Term)
(1 reference)
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北京 101111
Quick Overview for NFYB 抗体 (N-Term) (ABIN104771)
抗原
See all NFYB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 200-401-101
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Supplier
- Rockland
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原理
- NF-Y Antibody
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交叉反应 （详细）
- A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.
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产品特性
- Synonyms: rabbit anti-NF-Y Antibody, Nuclear transcription factor Y subunit B
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纯化方法
- This product was prepared from monospecific antiserum by a multi-step procedure which includes delipidation, salt fractionation and ion exchange chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Nuclear Transcription Factor Y, beta (NFYB) (AA 1-207) antibody
NFYB 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 6H6 unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (AA 1-207) antibodyNFYB 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 5D1 unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (AA 1-207) antibodyNFYB 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (N-Term) antibodyNFYB 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (AA 1-207) antibodyNFYB 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G10 unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (N-Term) antibodyNFYB 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) antibodyNFYB 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (N-Term) antibodyNFYB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (C-Term) antibodyNFYB 适用: 人, 小鼠, 大鼠, 犬, Pig, 豚鼠, 兔, 小鸡, 猴, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Transcription Factor Y, beta (NFYB) (N-Term) antibodyNFYB 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig, 豚鼠, 兔, 小鸡, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Gel Shift Dilution: 2.0 to 4.0 μL
Application Note: This product was assayed by immunoblot and found to be reactive against the 25 kDa B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (B subunit specific). Minimal reaction was observed by immunoblot against the 35 kDa A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 μL per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#611-1302) Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS substrate (#ABTS-100). A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, (2008) (PubMed).
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: "Posttranslational regulation of NF-YA modulates NF-Y transcriptional activity." in: Molecular biology of the cell, Vol. 19, Issue 12, pp. 5203-13, (2008) (PubMed).
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- NFYB (Nuclear Transcription Factor Y, beta (NFYB))
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别名
- NFYB
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背景
- Background: Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
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基因ID
- 4801
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UniProt
- P25208
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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