NYS48/HAUS8 抗体 (pSer70)
(1 reference)
Quick Overview for NYS48/HAUS8 抗体 (pSer70) (ABIN1043900)
抗原
See all NYS48/HAUS8 (HAUS8) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer70
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Supplier Product No.
- 600-401-c52
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Supplier
- Rockland
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原理
- HICE1 phosphoS70 Antibody
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交叉反应 （详细）
- Hice1 pS70 affinity purified antibody is directed against the phosphorylated form of human Hice1 protein at the S70 residue.
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产品特性
- Synonyms: rabbit anti-HICE1 pS70 antibody, HICE-1, HICE 1, HAUS8, HAUS-8, HAUS 8, HAUS augmin-like complex subunit 8, HEC1/NDC80-interacting centrosome-associated protein 1, Sarcoma antigen NY-SAR-48
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Hice1 pS70 Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) antibody
KO Validated HAUS8 适用: 人 WB, IF, ICC, IHC (p), IP 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) (AA 166-410) antibodyHAUS8 适用: 人 WB, ELISA, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) (AA 1-248) antibodyHAUS8 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) (Ser70) antibodyHAUS8 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) antibodyHAUS8 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) antibodyHAUS8 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) (AA 57-106) antibodyHAUS8 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-HAUS Augmin-Like Complex, Subunit 8 (HAUS8) (N-Term) antibodyHAUS8 适用: 人, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Hice1 pS70 antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kDa in size corresponding to human phosphorylated Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Western Blot Dilution: 1.22 μg/mL
ELISA Dilution: 1:17,000-1:45,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.22 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Hice1, a novel microtubule-associated protein required for maintenance of spindle integrity and chromosomal stability in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3652-62, (2008) (PubMed).
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: "Hice1, a novel microtubule-associated protein required for maintenance of spindle integrity and chromosomal stability in human cells." in: Molecular and cellular biology, Vol. 28, Issue 11, pp. 3652-62, (2008) (PubMed).
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- NYS48/HAUS8 (HAUS8) (HAUS Augmin-Like Complex, Subunit 8 (HAUS8))
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别名
- HAUS8
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背景
- Background: Hice1 pS70 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the gamma TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.
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基因ID
- 93323
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NCBI登录号
- NP_219485
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UniProt
- Q9BT25
抗原
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