GST 抗体 (Rhodamine)
Quick Overview for GST 抗体 (Rhodamine) (ABIN1043858)
抗原
See all GST 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-400-200
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Supplier
- Rockland
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原理
- GST Antibody Rhodamine Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
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产品特性
- Synonyms: rabbit anti-GST antibody Rhodamine conjugation, TRITC conjugated rabbit anti-GST antibody, Glutathione-S-Transferase
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纯化方法
- GST Antibody Rhodamine Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.
Immunogen Type: Native Protein
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亚型
- IgG
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Labeling Ratio
- 2.4
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anti-Glutathione S Transferase (GST) antibody
GST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibody (Biotin)GST 适用: 化学剂 WB, ELISA 宿主: 小鼠 Monoclonal vpg66 Biotin
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP 宿主: 小鼠 Monoclonal 2F10B9 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP, DB 宿主: 小鼠 Monoclonal GST-B6 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Nicotiana tabacum WB 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB, ELISA, IP, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 6G9C6 unconjugated
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应用备注
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Flow Cytometry Dilution: User Optimized
Application Note: GST Antibody Rhodamine Conjugated has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
FLISA Dilution: User Optimized
IF Microscopy Dilution: 1:500-1:2,500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store GST Antibody Rhodamine Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This GST Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GST (Glutathione S Transferase (GST))
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别名
- GST
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背景
- Background: GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
抗原
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