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Myc Tag 抗体 (Biotin)

WB, IP, ELISA, IHC 宿主: 兔 Polyclonal Biotin
产品编号 ABIN104384
发货至: 中国
  • 抗原 See all Myc Tag products
    Myc Tag
    适用
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    标记
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    This Myc Tag antibody is conjugated to Biotin
    应用范围
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    Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)
    序列
    EQKLISEEDL
    特异性
    This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    免疫原
    This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
    亚型
    IgG
  • 应用备注
    Suggested dilutions:
    ELISA : 1:100,000 – 1:500,000
    Western Blot : 1:2,000 - 1:10,000
    Immunohistochemistry: 1:1,000 - 1:5,000
    Other Dilution: User Optimized
    限制
    仅限研究用
  • 状态
    Lyophilized
    浓度
    1.0 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    tore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ° C as an undiluted liquid. Dilute only prior to immediate use.
  • 抗原
    Myc Tag
    Abstract
    Myc Tag 产品
    物质类
    Tag
    背景
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
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