Myc Tag 抗体 (Biotin)
Quick Overview for Myc Tag 抗体 (Biotin) (ABIN104384)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-406-381
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Supplier
- Rockland
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序列
- EQKLISEEDL
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特异性
- This affinity purified antibody is directed against human c-myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.
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产品特性
- Concentration Definition: by UV absorbance at 280 nm
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免疫原
- This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
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亚型
- IgG
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anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 兔 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (AA 408-439) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Myc Tag (AA 408-439) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: Human Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (C-Term) antibody适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 unconjugated
anti-Myc Tag (C-Term) antibody (Biotin)适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 Biotin
anti-Myc Tag (C-Term) antibody (FITC)适用: 人 FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 FITC
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated
anti-Myc Tag (AA 408-439) antibodyWB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated
anti-Myc Tag (AA 410-419) antibodyWB 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Suggested dilutions:
ELISA : 1:100,000 – 1:500,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized -
限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- 1.0 mg/mL
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缓冲液
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free -
储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
- tore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 ° C as an undiluted liquid. Dilute only prior to immediate use.
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- Myc Tag
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物质类
- Tag
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背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
抗原
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