IL-27 抗体
(1 reference)
Quick Overview for IL-27 抗体 (ABIN1043835)
抗原
See all IL-27 (IL27) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 210-501-b54
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Supplier
- Rockland
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原理
- Mouse IL-27/p28 Antibody
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交叉反应 （详细）
- This antibody is specific for mouse and rat p28 protein.
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产品特性
- Synonyms: rat anti-IL-27/p28 antibody, rat anti-interleukin 27a antibody, Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30
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纯化方法
- IL-27 / p28 antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-IL-27/p28 monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.
Immunogen Type: Recombinant Protein
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亚型
- IgG2a kappa
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anti-Interleukin 27 (IL27) (AA 177-243) antibody
IL27 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 3F12 unconjugated
anti-Interleukin 27 (IL27) (C-Term) antibodyIL27 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 27 (IL27) (AA 1-243) antibodyIL27 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 27 (IL27) (AA 1-243) antibodyIL27 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin 27 (IL27) (N-Term) antibodyIL27 适用: 人, 小鼠 WB, IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 27 (IL27) (AA 57-87) antibodyIL27 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB3263 unconjugated
anti-Interleukin 27 (IL27) (AA 29-243) antibodyIL27 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 27 (IL27) (AA 31-46) antibodyIL27 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 27 (IL27) antibodyIL27 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal B-G49 unconjugated
anti-Interleukin 27 (IL27) (C-Term) antibodyIL27 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry Dilution: 1 μg / ml
Application Note: IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in Flow Cytometry, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.
Western Blot Dilution: 1:1000
ELISA Dilution: 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11495-500, (2010) (PubMed).
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: "Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11495-500, (2010) (PubMed).
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- IL-27 (IL27) (Interleukin 27 (IL27))
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别名
- Il27
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背景
- Background: Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.
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基因ID
- 246779
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NCBI登录号
- NP_663611
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UniProt
- Q8K3I6
抗原
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