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Myc Tag 抗体

Name: Myc Tag 抗体
SKU: ABIN104383
Availability: OutOfStock

This anti-Myc Tag antibody is a 兔多克隆 antibody detecting Myc Tag in WB, IP 和 ELISA. Suitable for . This Primary Antibody has been cited in 3+ publications.

Git1 phosphorylation at Tyr-554 weakened its association with Hic-5. (Myc Tag 抗体)

Git1 phosphorylation at Tyr-554 was enhanced by co-expression of paxillin. (Myc Tag 抗体)

2 images

(3 references)

产品编号 ABIN104383

Supplier Product No.: 600-401-381

发货至: 中国

Datasheet as PDF

Quick Overview for Myc Tag 抗体 (ABIN104383)

抗原

Myc Tag

适用

请咨询

宿主

兔

克隆类型

多克隆

标记

This Myc Tag antibody is un-conjugated

应用范围

Western Blotting (WB), Immunoprecipitation (IP), ELISA

Supplier Product No.

600-401-381

Supplier

Rockland

序列

EQKLISEEDL

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

This antibody was purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide, E-Q-K-L-I-S-E-E-D-L, conjugated to KLH using maleimide. The sequence corresponds to amino acids 410-419 of human c-Myc.

亚型

IgG
anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 兔 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody

anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody

anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal unconjugated

anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: Human Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody

anti-Myc Tag (C-Term) antibody
适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 unconjugated

anti-Myc Tag (C-Term) antibody (Biotin)
适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), ICC, FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 Biotin

anti-Myc Tag (C-Term) antibody (FITC)
适用: 人 FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 FITC

anti-Myc Tag antibody
WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA 宿主: 小鼠 Monoclonal 9E10- unconjugated

anti-Myc Tag (AA 410-419) antibody
WB 宿主: 山羊 Polyclonal unconjugated

应用备注

Anti-Myc has utility to detect the fusion protein of the Myc epitope cloned along with the target gene. As such, anti-Myc/Myc can be used to identify fusion proteins containing the Myc epitope. The antibody recognizes the Myc tag fused either to the AMINO- or CARBOXY- termini of targeted proteins. This antibody is suitable for ELISA and western blotting and was tested against both the immunizing peptide and Myc-tagged recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Yue, Nabar, Shi, Kamenyeva, Xiao, Hwang, Wang, Kehrl: "SARS-Coronavirus Open Reading Frame-3a drives multimodal necrotic cell death." in: Cell death & disease, Vol. 9, Issue 9, pp. 904, (2018) (PubMed).

Fujikawa, Matsumoto, Kuboyama, Suzuki, Noda: "Specific dephosphorylation at tyr-554 of git1 by ptprz promotes its association with paxillin and hic-5." in: PLoS ONE, Vol. 10, Issue 3, pp. e0119361, (2016) (PubMed).

Sorensen, Conner: "γ-secretase-dependent cleavage initiates notch signaling from the plasma membrane." in: Traffic (Copenhagen, Denmark), Vol. 11, Issue 9, pp. 1234-45, (2011) (PubMed).
抗原

Myc Tag

物质类

Tag

背景

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.