IL-33 抗体 (Biotin)
(1 reference)
Quick Overview for IL-33 抗体 (Biotin) (ABIN1043805)
抗原
See all IL-33 (IL33) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 209-406-b50
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Supplier
- Rockland
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原理
- IL-33 Antibody Biotin Conjugated
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交叉反应 （详细）
- In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids.
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产品特性
- Synonyms: rabbit anti-Interleukin-33 biotin conjugated antibody, rabbit anti-IL-33 biotin conjugated antibody, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV
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纯化方法
- This purified antibody has been heated to 56°C for 30 minutes.
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免疫原
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Interleukin 33 (IL33) (AA 112-270) antibody
IL33 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C11 unconjugated
anti-Interleukin 33 (IL33) antibodyIL33 适用: 人 WB, ELISA, IHC, IP, Func 宿主: 小鼠 Monoclonal IL33305B unconjugated
anti-Interleukin 33 (IL33) (AA 110-261) antibodyIL33 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 2# unconjugated
anti-Interleukin 33 (IL33) (AA 185-264) antibodyIL33 适用: 小鼠, 大鼠 ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 33 (IL33) (AA 171-269) antibodyIL33 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D8 unconjugated
anti-Interleukin 33 (IL33) (AA 102-264) antibodyIL33 适用: 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 33 (IL33) antibodyIL33 适用: 人 WB, IHC (p), EIA, Func 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 33 (IL33) antibody (Biotin)IL33 适用: 人 WB, EIA 宿主: 兔 Polyclonal Biotin
anti-Interleukin 33 (IL33) (AA 1-111) antibodyIL33 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 33 (IL33) antibodyIL33 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: This purified antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 18 kDa in size corresponding to mature human IL-32α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:2,000-1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Mature interleukin-33 is produced by calpain-mediated cleavage in vivo." in: Biochemical and biophysical research communications, Vol. 387, Issue 1, pp. 218-22, (2009) (PubMed).
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: "Mature interleukin-33 is produced by calpain-mediated cleavage in vivo." in: Biochemical and biophysical research communications, Vol. 387, Issue 1, pp. 218-22, (2009) (PubMed).
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- IL-33 (IL33) (Interleukin 33 (IL33))
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别名
- IL33
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背景
- Background: IL-33 (also known as Interleukin-33, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV) is a cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL-33 induces T helper type 2-associated cytokines. IL-33 is a secreted cytokine that is expressed at high levels in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes and is almost undetectable in placenta. The 31 kDa precursor is proteolytically converted to an 18 kDa mature form by CASP1.
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基因ID
- 90865, 15559209
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UniProt
- O95760
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途径
- Production of Molecular Mediator of Immune Response
抗原
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