IL32 抗体 (Biotin)
(1 reference)
Quick Overview for IL32 抗体 (Biotin) (ABIN1043804)
抗原
See all IL32 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 209-406-b49
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Supplier
- Rockland
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原理
- IL-32A Antibody Biotin Conjugated
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交叉反应 （详细）
- In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-32A in cell supernatants and certain body fluids.
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产品特性
- Synonyms: rabbit anti-IL-32A biotin conjugated antibody, rabbit anti-interleukin-32A biotin conjugated antibody, Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32α, Interleukin-32, IL-32 isoform 4
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纯化方法
- This purified antibody has been heated to 56°C for 30 minutes.
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免疫原
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Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-32A protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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Labeling Ratio
- 10-20
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anti-Interleukin 32 (IL32) (AA 1-188) antibody
IL32 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 32 (IL32) (AA 151-234) antibodyIL32 适用: 人 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 32 (IL32) (AA 31-234) antibodyIL32 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 32 (IL32) (C-Term) antibodyIL32 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 32 (IL32) antibodyIL32 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 32 (IL32) (AA 1-188) antibodyIL32 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin 32 (IL32) (AA 1-131) antibodyIL32 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal AT2F9 unconjugated
anti-Interleukin 32 (IL32) (AA 1-131), (N-Term) antibodyIL32 适用: 人 WB, EIA 宿主: 小鼠 Monoclonal AT2F9 unconjugated
anti-Interleukin 32 (IL32) (C-Term) antibodyIL32 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 32 (IL32) (C-Term) antibodyIL32 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kDa in size corresponding to native human IL-32α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:2,000-1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Interleukin-32 expression in the pancreas." in: The Journal of biological chemistry, Vol. 284, Issue 26, pp. 17868-76, (2009) (PubMed).
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: "Interleukin-32 expression in the pancreas." in: The Journal of biological chemistry, Vol. 284, Issue 26, pp. 17868-76, (2009) (PubMed).
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- IL32 (Interleukin 32 (IL32))
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别名
- IL32
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背景
- Background: IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32α, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFα and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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基因ID
- 9235, 61658634
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UniProt
- P24001
抗原
-
