CCL4 抗体 (HRP)
(1 reference)
Quick Overview for CCL4 抗体 (HRP) (ABIN1043803)
抗原
See all CCL4 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 209-403-b97
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Supplier
- Rockland
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原理
- MIP-1 beta Antibody Peroxidase Conjugated
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产品特性
- Synonyms: rabbit anti-MIP-1 beta Antibody Peroxidase Conjugated, rabbit anti-MIP-1b Antibody HRP Conjugated, CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, T-cell activation protein 2, Protein H400, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, MIP-1ß
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纯化方法
- Human MIP-1 beta Antibody Peroxidase Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.
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免疫原
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Immunogen: MIP-1 beta Antibody Peroxidase Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1ß protein.
Immunogen Type: Recombinant Protein
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亚型
- IgG
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anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) antibody
CCL4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) antibodyCCL4 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 7C9E4 unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) antibodyCCL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 8F1C2 unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 26-92) antibodyCCL4 适用: 人, 小鼠, 兔 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (Internal Region) antibodyCCL4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) antibodyCCL4 适用: 人 WB, ELISA, IHC (p), Func 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (C-Term) antibodyCCL4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) antibodyCCL4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 22-48) antibodyCCL4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Ligand 4 (CCL4) (AA 24-92) antibodyCCL4 适用: 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This protein-A purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15 kDa in size corresponding to human MIP-1ß protein by western blotting in the appropriate cell lysate or extract. Immunology Research
Western Blot Dilution: 1:500
ELISA Dilution: 1:10,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store MIP-1 beta Antibody Peroxidase Conjugated at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Natural truncation of the chemokine MIP-1 beta /CCL4 affects receptor specificity but not anti-HIV-1 activity." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 32348-52, (2002) (PubMed).
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: "Natural truncation of the chemokine MIP-1 beta /CCL4 affects receptor specificity but not anti-HIV-1 activity." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 32348-52, (2002) (PubMed).
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- CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
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别名
- CCL4
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背景
- Background: MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated. MIP-1 beta Antibody Peroxidase Conjugated is useful for researchers interested in Immunology Research.
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基因ID
- 6351
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NCBI登录号
- NP_002975
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UniProt
- P13236
抗原
-
