IL-25 抗体 (Biotin)
(1 reference)
Quick Overview for IL-25 抗体 (Biotin) (ABIN1043795)
抗原
See all IL-25 (IL25) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 209-306-c58
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Supplier
- Rockland
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原理
- IL-17E Biotin Conjugated Antibody
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交叉反应 （详细）
- This antibody is specific for human IL-17E protein.
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产品特性
- Synonyms: mouse Anti-IL-17E Biotin Conjugated Antibody, mouse anti-IL-25 biotin conjugated antibody, Interleukin 25, IL-25, Interleukin 17E, IL-17E
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纯化方法
- This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development.
Immunogen Type: Recombinant Protein
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亚型
- IgG1
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Labeling Ratio
- 10-20
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anti-Interleukin 25 (IL25) antibody
IL25 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 25 (IL25) (AA 32-148) antibodyIL25 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 25 (IL25) (Internal Region) antibodyIL25 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 25 (IL25) (AA 33-169) antibodyIL25 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 25 (IL25) (AA 76-104) antibodyIL25 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 25 (IL25) (AA 33-177) antibodyIL25 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 25 (IL25) (AA 1-177) antibodyIL25 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Interleukin 25 (IL25) antibody (PE)IL25 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal PE
anti-Interleukin 25 (IL25) (AA 115-132) antibodyIL25 适用: 人, 小鼠 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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Flow Cytometry Dilution: 1 μg/mL
Application Note: This purified antibody has been tested for use in Flow Cytometry and western blotting. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 0.5 μg/mL
ELISA Dilution: 1:10,000 - 1:50,000
Other: User optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Th17 cells and IL-17 receptor signaling are essential for mucosal host defense against oral candidiasis." in: The Journal of experimental medicine, Vol. 206, Issue 2, pp. 299-311, (2009) (PubMed).
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: "Th17 cells and IL-17 receptor signaling are essential for mucosal host defense against oral candidiasis." in: The Journal of experimental medicine, Vol. 206, Issue 2, pp. 299-311, (2009) (PubMed).
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- IL-25 (IL25) (Interleukin 25 (IL25))
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别名
- IL25
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背景
- Background: Anti-IL17E recognizes IL-17E (also known as IL25). IL-17E is produced and secreted by intra-epithelial lymphocytes, lung epithelial cells, alveolar macrophages, eosinophils, basophils, NKT cells, mast cells and cells of the gastro-intestinal tract and uterus. IL-17E stimulates the production of Th2-type cytokines. IL-17E limits Th17 cell development by inducing the expression of IL-13 by dendritic cells or by inhibiting IL-23 production by macrophages. Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. A number of cytokines, including IL-17E, have been implicated as oncogenes, reflecting the importance of these proteins in cell growth and survival. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and pro-inflammatory pathologies.
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基因ID
- 64806
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NCBI登录号
- NP_073626
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UniProt
- Q9H293
抗原
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