B-Phycoerythrin 抗体
Cited in 1 publication.
The 兔 多克隆 anti-B-Phycoerythrin antibody (ABIN1043760) specifically detects B-Phycoerythrin in WB, ELISA, IHC 和 DB.
The antibody is reactive with Red algae (Porphyridium cruentum) samples.
1 image
(1 reference)
产品编号 ABIN1043760
Supplier Product No.: 200-401-099
发货至:
中国
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Contact Info
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Quick Overview for B-Phycoerythrin 抗体 (ABIN1043760)
抗原
B-Phycoerythrin
(Phycoerythrin, B-)
适用
Red algae (Porphyridium cruentum)
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
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Supplier Product No.
- 200-401-099
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Supplier
- Rockland
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原理
- B-Phycoerythrin Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phycoerythrin [Porphyridium creuentum].
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产品特性
- Synonyms: rabbit anti-B-Phycoerythrin Antibody, rabbit anti B-Phycoerythrin, rabbit anti Phycoerythrin
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纯化方法
- Anti-B-Phycoerythrin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Phycoerythrin [Porphyridium creuentum]
Immunogen Type: Native Protein
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-B-Phycoerythrin antibody has been tested by ELISA and dot blot and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Capturing change in clonal composition amongst single mouse germinal centers." in: eLife, Vol. 7, (2018) (PubMed).
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: "Capturing change in clonal composition amongst single mouse germinal centers." in: eLife, Vol. 7, (2018) (PubMed).
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- B-Phycoerythrin (Phycoerythrin, B-)
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别名
- B-Phycoerythrin
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背景
- Background: Anti-B-Phycoerythrin Antibody detects the B-PE phycoerythrin protein. Phycoerythrin is a red protein from the light-harvesting phycobiliprotein family, present in cyanobacteria, and red algae. Phycoerythrin is organized in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. R-Phycoerythrin and B-Phycoerythrin are among the brightest biological fluorescent dyes and are commonly used as a fluorescent dye in many assays and is frequently conjugated to antibodies as a reporter molecule. The spectral properties of B-Phycoerythrin are absorption at 545 nm and emission at 572 nm.
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UniProt
- P11392
抗原
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