PDCD4 抗体 (pSer457) (Biotin)
(2 references)
Quick Overview for PDCD4 抗体 (pSer457) (Biotin) (ABIN1043755)
抗原
See all PDCD4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer457
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Supplier Product No.
- 200-306-964
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Supplier
- Rockland
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原理
- Pdcd4 phospho S457 Antibody Biotin Conjugated
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交叉反应 （详细）
- This antibody is specific for human Pdcd4 protein phosphorylated at Ser457.
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产品特性
- Synonyms: mouse anti-Pdcd4 pS457 Antibody biotin conjugation, biotin conjugated mouse anti-Pdcd4 pS457 Antibody, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody
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纯化方法
- Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography.
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免疫原
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Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG1 kappa
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anti-Programmed Cell Death 4 (PDCD4) (C-Term) antibody
PDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibodyPDCD4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-100) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibodyPDCD4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (N-Term) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer67) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) antibodyPDCD4 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) antibodyPDCD4 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Programmed Cell Death 4 (PDCD4) (AA 121-149) antibodyPDCD4 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB33836 unconjugated
anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibodyPDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9G6 unconjugated
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应用备注
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Application Note: Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody Biotin Conjugated has been tested by ELISA and western blotting and is suitable for immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Western Blot Dilution: 1:2,000 - 1:10,000
Immunoprecipitation Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).
: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, (2005) (PubMed).
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: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).
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- PDCD4 (Programmed Cell Death 4 (PDCD4))
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别名
- PDCD4
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背景
- Background: Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.
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基因ID
- 27250, 21735596
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UniProt
- Q53EL6
抗原
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