BIN1 抗体
(2 references)
Quick Overview for BIN1 抗体 (ABIN1043735)
抗原
See all BIN1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
Supplier Product No.
- 200-301-e60
-
Supplier
- Rockland
-
原理
- BIN1 Antibody
-
交叉反应 （详细）
- BIN1 antibody is specific for human BIN1 protein.
-
产品特性
- Synonyms: mouse anti-BIN1 Antibody, AMPHL, Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, BIN-1, BIN 1, BIN1 antibody
-
纯化方法
- Anti-BIN1 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: Anti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a fragment portion of recombinant human BIN1 protein followed by hybridoma development.
Immunogen Type: Recombinant Protein
-
亚型
- IgG2b
-
-
anti-Bridging Integrator 1 (BIN1) (AA 189-398) antibody
BIN1 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3B6F10 unconjugated
anti-Bridging Integrator 1 (BIN1) antibodyBIN1 适用: 人, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Bridging Integrator 1 (BIN1) (AA 230-409) antibodyBIN1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Bridging Integrator 1 (BIN1) antibodyBIN1 适用: 人 WB, IHC, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Bridging Integrator 1 (BIN1) (AA 189-398) antibodyBIN1 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3B6A4 unconjugated
anti-Bridging Integrator 1 (BIN1) (AA 414-593) antibodyBIN1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bridging Integrator 1 (BIN1) (AA 1-482) antibodyBIN1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Bridging Integrator 1 (BIN1) (AA 355-454) antibodyBIN1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C7 unconjugated
anti-Bridging Integrator 1 (BIN1) antibodyBIN1 适用: 小鼠 WB, IHC, ELISA, IP 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-Bridging Integrator 1 (BIN1) (AA 360-389) antibodyBIN1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Flow Cytometry Dilution: 0.5-1x10^6 cells
Immunohistochemistry Dilution: 1:100-1:500
Application Note: Anti-BIN1 antibody has been tested for use in ELISA, IP, and Western Blot. This antibody is suitable for use in IHC and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:500-1:1500
Immunoprecipitation Dilution: 10-100 μL
ELISA Dilution: 1:5000-1:50000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
-
: "Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy." in: Nature genetics, Vol. 39, Issue 9, pp. 1134-9, (2007) (PubMed).
: "Human T-cell lymphotrophic virus type I rex and p30 interactions govern the switch between virus latency and replication." in: The Journal of biological chemistry, Vol. 282, Issue 19, pp. 14608-15, (2007) (PubMed).
-
: "Mutations in amphiphysin 2 (BIN1) disrupt interaction with dynamin 2 and cause autosomal recessive centronuclear myopathy." in: Nature genetics, Vol. 39, Issue 9, pp. 1134-9, (2007) (PubMed).
-
- BIN1 (Bridging Integrator 1 (BIN1))
-
别名
- BIN1
-
背景
- Background: Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act as a cancer suppressor and inhibits malignant cell transformation. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Bin1 has also been implicated in Alzheimer disease and cardiac disease. Defects in Bin1 are the cause of centronuclear myopathy autosomal recessive, also known as autosomal recessive myotubular myopathy.
-
基因ID
- 274
-
NCBI登录号
- NP_004296
-
UniProt
- O00499
抗原
-
