DYKDDDDK Tag 抗体
(2 references)
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- 抗原 See all DYKDDDDK Tag products
- DYKDDDDK Tag
- 适用
- 请咨询
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This DYKDDDDK Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)
- Supplier Product No.
- 200-301-b13
- Supplier
- Rockland
- 原理
- DYKDDDDK Tag (Anti-FLAG®) Antibody
- 序列
- DYKDDDDK
- 交叉反应 （详细）
- The purified antibody is directed against the FLAG™ motif and is useful in determining its presence in various assays where the epitope tag is present at either the amino or carboxy terminus of recombinant proteins.
- 产品特性
- Synonyms: mouse anti-FLAG™ tag, Enterokinase Cleavage Site (ECS), mouse anti-DYKDDDDK, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys
- 纯化方法
- This product is an IgG fraction antibody purified from ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG™ epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide.
Immunogen Type: Conjugated Peptide
- 克隆位点
- 29E4-G7
- 亚型
- IgG2a kappa
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
anti-DYKDDDDK Tag antibody适用: 标记 WB, ELISA, IP, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated
anti-DYKDDDDK Tag antibody适用: 人 WB 宿主: 兔 Polyclonal RB0493 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A8E5 HRP
anti-DYKDDDDK Tag antibodyWB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibodyWB, IP, IF, IC 宿主: 小鼠 Monoclonal unconjugated
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- 应用备注
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 1:1,000-1:5,000
Application Note: Anti-FLAG antibody has been tested by ELISA and western blot and is optimally suited for monitoring the expression of FLAG™ tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG™ epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag.
Western Blot Dilution: 1:2,000-1:10,000
ELISA Dilution: 1:150,000 - 1:250,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Phospho-?Np63? is a key regulator of the cisplatin-induced microRNAome in cancer cells." in: Cell death and differentiation, Vol. 18, Issue 7, pp. 1220-30, (2011) (PubMed).
: "Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells." in: BioTechniques, Vol. 20, Issue 1, pp. 136-41, (1996) (PubMed).
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: "Phospho-?Np63? is a key regulator of the cisplatin-induced microRNAome in cancer cells." in: Cell death and differentiation, Vol. 18, Issue 7, pp. 1220-30, (2011) (PubMed).
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- 抗原
- DYKDDDDK Tag
- 别名
- FLAG (DYKDDDDK Tag 产品)
- 物质类
- Tag
- 背景
- Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
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