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aHSP 抗体

Cited in 1 publication. This anti-aHSP antibody is a 兔 多克隆 antibody detecting aHSP in WB, IHC 和 ELISA. Suitable for 人 和 小鼠.
Rockland
产品编号 ABIN1043695
Supplier Product No.: 100-401-e79
发货至: 中国
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中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for aHSP 抗体 (ABIN1043695)

抗原

See all aHSP 抗体
aHSP (alpha Hemoglobin Stabilizing Protein (aHSP))

适用

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人, 小鼠

宿主

  • 37
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克隆类型

  • 38
  • 5
多克隆

标记

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This aHSP antibody is un-conjugated

应用范围

  • 34
  • 15
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA
  • Supplier Product No.

    100-401-e79

    Supplier

    Rockland

    原理

    AHSP Antibody

    交叉反应 (详细)

    Anti-AHSP Antibody is directed against the human AHSP protein.

    产品特性

    Synonyms: rabbit anti-AHSP antibody, Alpha-hemoglobin-stabilizing protein (AHSP), Erythroid differentiation-related factor, Erythroid-associated factor, EDRF, ERAF

    纯化方法

    The product was prepared from monospecific antiserum by delipidation and defibrination.

    免疫原

    Immunogen: Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein.

    Immunogen Type: Recombinant Protein

  • 应用备注

    Immunohistochemistry Dilution: 1:8000

    Application Note: Anti-AHSP Antibody has been tested in western blot and immunohistochemistry and is suitable for immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures and ELISA. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: 1:500-1:1000

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度

    86 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • Nasimuzzaman, Khandros, Wang, Kong, Zhao, Weiss, Rivella, Weiss, Persons: "Analysis of alpha hemoglobin stabilizing protein overexpression in murine β-thalassemia." in: American journal of hematology, Vol. 85, Issue 10, pp. 820-2, (2010) (PubMed).

  • 抗原

    aHSP (alpha Hemoglobin Stabilizing Protein (aHSP))

    别名

    AHSP

    背景

    Background: AHSP Antibody detects Alpha hemoglobin stabilizing protein (AHSP). AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. AHSP binds free a-globin to promote its folding and inhibit its ability to produce damaging reactive oxygen species. Reduced AHSP levels correlate with increased severity of b-thalassemia in some human cohorts.

    基因ID

    51327

    NCBI登录号

    NP_057717

    UniProt

    Q9NZD4
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