aHSP 抗体
(1 reference)
Quick Overview for aHSP 抗体 (ABIN1043695)
抗原
See all aHSP 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 100-401-e79
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Supplier
- Rockland
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原理
- AHSP Antibody
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交叉反应 （详细）
- Anti-AHSP Antibody is directed against the human AHSP protein.
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产品特性
- Synonyms: rabbit anti-AHSP antibody, Alpha-hemoglobin-stabilizing protein (AHSP), Erythroid differentiation-related factor, Erythroid-associated factor, EDRF, ERAF
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纯化方法
- The product was prepared from monospecific antiserum by delipidation and defibrination.
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免疫原
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Immunogen: Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein.
Immunogen Type: Recombinant Protein
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anti-alpha Hemoglobin Stabilizing Protein (aHSP) (AA 2-102) antibody
aHSP 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha Hemoglobin Stabilizing Protein (aHSP) antibodyaHSP 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-alpha Hemoglobin Stabilizing Protein (aHSP) (AA 1-102) antibodyaHSP 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-alpha Hemoglobin Stabilizing Protein (aHSP) (AA 1-102) antibodyaHSP 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3C9 unconjugated
anti-alpha Hemoglobin Stabilizing Protein (aHSP) (AA 1-102) antibodyaHSP 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-alpha Hemoglobin Stabilizing Protein (aHSP) (AA 1-102) antibodyaHSP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha Hemoglobin Stabilizing Protein (aHSP) (AA 2-102) antibodyaHSP 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha Hemoglobin Stabilizing Protein (aHSP) (AA 1-102) antibodyaHSP 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-alpha Hemoglobin Stabilizing Protein (aHSP) (AA 2-102) antibodyaHSP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:8000
Application Note: Anti-AHSP Antibody has been tested in western blot and immunohistochemistry and is suitable for immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures and ELISA. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:500-1:1000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 86 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Analysis of alpha hemoglobin stabilizing protein overexpression in murine β-thalassemia." in: American journal of hematology, Vol. 85, Issue 10, pp. 820-2, (2010) (PubMed).
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: "Analysis of alpha hemoglobin stabilizing protein overexpression in murine β-thalassemia." in: American journal of hematology, Vol. 85, Issue 10, pp. 820-2, (2010) (PubMed).
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- aHSP (alpha Hemoglobin Stabilizing Protein (aHSP))
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别名
- AHSP
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背景
- Background: AHSP Antibody detects Alpha hemoglobin stabilizing protein (AHSP). AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. AHSP binds free a-globin to promote its folding and inhibit its ability to produce damaging reactive oxygen species. Reduced AHSP levels correlate with increased severity of b-thalassemia in some human cohorts.
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基因ID
- 51327
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NCBI登录号
- NP_057717
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UniProt
- Q9NZD4
抗原
-
