VaChT Transporter (AA 521-532) 抗体
适用: 人
IHC, WB, IF/ICC
宿主: 小鼠
Monoclonal
S6-38
unconjugated
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- 抗原
- VaChT Transporter
- 抗原表位
- AA 521-532
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适用
- 人
-
宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)
- 原理
- Anti-VAChT Transporter Mouse Monoclonal Antibody
- 特异性
- This antibody recognizes human, mouse, and rat VAChT.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Purified by Protein G affinity chromatography.
- 免疫原
- Synthetic peptide corresponding to aa 521-532 (CEDDYNYYYTRS) of human VAChT (accession no. NP_003046)
- 克隆位点
- S6-38
- 亚型
- IgG1
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-
- 应用备注
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Immunoblotting: use at 1-10 μg/mL. A band of 55 kDa is detected. The molecular weight of glycosylated VAChT is 70 kDa.
Immunohistochemistry: use at 1-10 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: rat brain - 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Dilute in PBS or medium that is identical to that used in the assay system.
- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
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- 抗原
- VaChT Transporter
- 背景
- Vesicular acetylcholine transporter,Involved in acetylcholine transport into synaptic vesicles. {PubMed:8071310}.,VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.,Membrane, Multi-pass membrane protein,VAChT, Solute carrier family 18 member 3
- UniProt
- Q16572
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