VaChT Transporter (AA 521-532) 抗体
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Quick Overview for VaChT Transporter (AA 521-532) 抗体 (ABIN1043018)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 521-532
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原理
- Anti-VAChT Transporter Mouse Monoclonal Antibody
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特异性
- This antibody recognizes human, mouse, and rat VAChT.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified by Protein G affinity chromatography.
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免疫原
- Synthetic peptide corresponding to aa 521-532 (CEDDYNYYYTRS) of human VAChT (accession no. NP_003046)
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亚型
- IgG1
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应用备注
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Immunoblotting: use at 1-10 μg/mL. A band of 55 kDa is detected. The molecular weight of glycosylated VAChT is 70 kDa.
Immunohistochemistry: use at 1-10 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their applications.
Positive control: rat brain -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Dilute in PBS or medium that is identical to that used in the assay system.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
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- VaChT Transporter
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背景
- Vesicular acetylcholine transporter,Involved in acetylcholine transport into synaptic vesicles. {PubMed:8071310}.,VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.,Membrane, Multi-pass membrane protein,VAChT, Solute carrier family 18 member 3
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UniProt
- Q16572
抗原
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