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VaChT Transporter (AA 521-532) 抗体

The 小鼠 单克隆 anti-VaChT Transporter antibody is suitable to detect VaChT Transporter in samples from 人. It has been validated for IHC, WB 和 IF/ICC.
产品编号 ABIN1043018
发货至: 中国
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Quick Overview for VaChT Transporter (AA 521-532) 抗体 (ABIN1043018)

抗原

VaChT Transporter

适用

  • 1
  • 1
  • 1

宿主

  • 1
小鼠

克隆类型

  • 1
单克隆

标记

  • 1
非结合性

应用范围

Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

克隆位点

S6-38
  • 抗原表位

    AA 521-532

    原理

    Anti-VAChT Transporter Mouse Monoclonal Antibody

    特异性

    This antibody recognizes human, mouse, and rat VAChT.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified by Protein G affinity chromatography.

    免疫原

    Synthetic peptide corresponding to aa 521-532 (CEDDYNYYYTRS) of human VAChT (accession no. NP_003046)

    亚型

    IgG1
  • 应用备注

    Immunoblotting: use at 1-10 μg/mL. A band of 55 kDa is detected. The molecular weight of glycosylated VAChT is 70 kDa.
    Immunohistochemistry: use at 1-10 μg/mL.
    These are recommended concentrations.
    Enduser should determine optimal concentrations for their applications.
    Positive control: rat brain

    限制

    仅限研究用
  • 状态

    Liquid

    溶解方式

    Dilute in PBS or medium that is identical to that used in the assay system.

    浓度

    1.0 mg/mL

    缓冲液

    PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
  • 抗原

    VaChT Transporter

    背景

    Vesicular acetylcholine transporter,Involved in acetylcholine transport into synaptic vesicles. {PubMed:8071310}.,VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.,Membrane, Multi-pass membrane protein,VAChT, Solute carrier family 18 member 3

    UniProt

    Q16572
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