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Vimentin 抗体 (HRP)

Cited in 5+ publications. The HRP-conjugated 小鼠 单克隆 anti-Vimentin antibody (Clone RV202) (ABIN1042494) specifically detects Vimentin in FACS, ICC, IHC (fro), IHC (p) 和 WB. The antibody is reactive with Various Species samples.
产品编号 ABIN1042494
发货至: 中国
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Quick Overview for Vimentin 抗体 (HRP) (ABIN1042494)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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Various Species

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小鼠

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单克隆

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This Vimentin antibody is conjugated to HRP

应用范围

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Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

克隆位点

RV202
  • 原理

    Mouse anti Vimentin, conjugated with HRP

    特异性

    RV202-HRP reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchyme derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-HRP is suitable for immunoblotting, immunocytochemistry on cell cultures and cytospins, as well as immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended dilution is 1:10.

    产品特性

    Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

    免疫原

    RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

    亚型

    IgG1
  • 限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Each vial contains 1 mL sterile filtered HRP-conjugated vimentin monoclonal antibody in PBS containing 0.1 % BSA. Approximately 100 tests.

    储存条件

    4 °C,-20 °C

    储存方法

    The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
  • Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

    Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

    Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

    Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, (1988) (PubMed).

    Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, (1987) (PubMed).

  • 抗原

    Vimentin (VIM)

    别名

    Vimentin

    UniProt

    P08670

    途径

    Caspase Cascade in Apoptosis
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