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Vimentin 抗体 (FITC)

There are 5+ publications for this product available. The 小鼠 单克隆 anti-Vimentin antibody is suitable to detect Vimentin in samples from Various Species. It has been validated for FACS, ICC, IHC (fro), IHC (p) 和 WB.
Pubmed (5 references)
产品编号 ABIN1042493
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Vimentin 抗体 (FITC) (ABIN1042493)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

适用

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Various Species

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小鼠

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单克隆

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This Vimentin antibody is conjugated to FITC

应用范围

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Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

克隆位点

RV202
Discover our top product VIM Primary Antibody

  • 原理

    Mouse anti Vimentin, conjugated with FITC

    特异性

    RV202-FITC reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-FITC is suitable for immunocytochemistry on cell cultures and cytospins, immunohistochemistry on frozen tissues, as well as for flow cytometry. Optimal antibody dilution should be determined by titration, recommended dilution is 1:10.

    产品特性

    Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

    免疫原

    RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

    亚型

    IgG1
  • anti-Vimentin (VIM) (AA 371-466) antibody
    anti-Vimentin (VIM) (AA 371-466) antibody

    KO Validated VIM 适用: 人 WB, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

    anti-Vimentin (VIM) (C-Term) antibody
    anti-Vimentin (VIM) (C-Term) antibody

    VIM 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

    anti-Vimentin (VIM) antibody
    anti-Vimentin (VIM) antibody

    KO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

    anti-Vimentin (VIM) antibody
    anti-Vimentin (VIM) antibody

    VIM 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB41034 unconjugated

    anti-Vimentin (VIM) (AA 1-200) antibody
    anti-Vimentin (VIM) (AA 1-200) antibody

    VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

    anti-Vimentin (VIM) antibody
    anti-Vimentin (VIM) antibody

    VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

    anti-Vimentin (VIM) antibody
    anti-Vimentin (VIM) antibody

    VIM 适用: 人, 大鼠, 小鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

    anti-Vimentin (VIM) antibody
    anti-Vimentin (VIM) antibody

    VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS 宿主: 小鼠 Monoclonal VM452 unconjugated

    anti-Vimentin (VIM) antibody
    anti-Vimentin (VIM) antibody

    VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 马 WB, IHC, IF, FACS 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

    anti-Vimentin (VIM) (AA 325-375) antibody
    anti-Vimentin (VIM) (AA 325-375) antibody

    VIM 适用: 人, 小鼠 WB, IHC, ICC, IF (p) 宿主: 兔 Monoclonal BLR100G unconjugated Recombinant Antibody

  • 限制

    仅限研究用

  • 状态

    Liquid

    缓冲液

    Each vial contains 1 mL FITC-conjugated vimentin monoclonal antibody in PBS containing 0,1 % BSA, 0.09 % sodium azide. Approximately 100 tests.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
  • Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

    Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

    Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

    Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, (1988) (PubMed).

    Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, (1987) (PubMed).

  • 抗原

    Vimentin (VIM)

    别名

    Vimentin

    UniProt

    P08670

    途径

    Caspase Cascade in Apoptosis
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