(3 references)-
- 抗原 See all CD43 (SPN) 抗体
- CD43 (SPN) (Sialophorin (SPN))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD43 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Clone MT1 produces mouse IgG1 immunoglobulins reactive with a 95-115 kD highly sialated glycoprotein. This antibody is used to identify B cell lines and myeloma cells. It may be used in the diagnosis of chronic lymphocytic leukemias (CLL), as an alternative to CD5. The antigen detected by MT1 is the only marker expressed on neoplasms of the very early precursor cells. In immunohistochemistry it reacts with T cells, macrophages, myeloid cells and B cells (weak). It is used for the typing of lymphomas in paraffin sections. CD43 may function as an adhesion molecule via interaction with CD54 although this has not been definitely established. It may also inhibit leucocyte interactions with other cells. The antigen is involved in the activation of T-cells, B cells, NK cells and monocytes. The membrane proximal portion of the cytoplasmic domain mediates an association with the cytoskeleton.
- 产品特性
- CD markers
- 纯化方法
- Purified
- 克隆位点
- MT1
- 亚型
- IgG1
- Top Product
- Discover our top product SPN Primary Antibody
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anti-Sialophorin (SPN) antibody
SPN 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal DF-T1 unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-59 unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC (p), IP, Func 宿主: 小鼠 Monoclonal MEM-59 unconjugated
anti-Sialophorin (SPN) (AA 276-400) antibodySPN 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC, IF, StM 宿主: 兔 Monoclonal SPN-1766R unconjugated Recombinant Antibody
anti-Sialophorin (SPN) antibodySPN 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal rSPN-839 unconjugated Recombinant Antibody
anti-Sialophorin (SPN) antibodySPN 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sialophorin (SPN) (AA 1-400) antibodySPN 适用: 人 FACS, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Sialophorin (SPN) antibody (FITC)SPN 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-59 FITC
anti-Sialophorin (SPN) antibody (Biotin)SPN 适用: 人 FACS, WB, IHC (p), IP 宿主: 小鼠 Monoclonal MEM-59 Biotin
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- 应用备注
- The antibody is suitable for flow cytometry for analysis of blood and bone marrow samples or in immunohistochemistry using cytospots, paraffin or frozen tissue sections. Optimal antibody dilution should be determined by titration.
- 说明
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MT1 is a monoclonal antibody derived by fusion of X63 mouse myeloma cells with spleen cells from of Balb/c mice, intraperitoneally immunized with human monocytes isolated by centrifugal elutriation. Immunizations were carried out at least twice at 2-week intervals with: A) cell suspensions of a lymph node involved by Hodgkin's disease, B) cell suspensions of a lymph node involved by Bchronic lymphatic leukemia, C) cells of Hodgkin's cell line DEV, respectively. No adjuvants were used (3).
- 限制
- 仅限研究用
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- 缓冲液
- PBS containing 0.09% sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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: "Negative regulation of T-cell adhesion and activation by CD43." in: Nature, Vol. 377, Issue 6549, pp. 535-8, (1995) (PubMed).
: "Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 2, pp. 663-7, (1992) (PubMed).
: "Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections." in: The American journal of pathology, Vol. 127, Issue 3, pp. 418-29, (1987) (PubMed).
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: "Negative regulation of T-cell adhesion and activation by CD43." in: Nature, Vol. 377, Issue 6549, pp. 535-8, (1995) (PubMed).
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- 抗原
- CD43 (SPN) (Sialophorin (SPN))
- 别名
- CD43 (SPN 产品)
- 别名
- CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody
- 背景
- CD43 antigen is expressed on all T cells, NK cells, myeloid cells and monocytes as well as on CD5 positive mature B cells. It is also present on all immature hematopoietic cells in the bone marrow. The antigen is deficient in patients with Wiskott-Aldrich syndrome. A soluble form of CD43 is present in human serum.
- 途径
- Regulation of Leukocyte Mediated Immunity
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