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Merlin 抗体 (pSer518)

Name: Merlin 抗体 (pSer518)
SKU: ABIN104130
Availability: OutOfStock

This anti-Merlin antibody is a 兔多克隆 antibody detecting Merlin in WB, ELISA 和 IHC. Suitable for 小鼠. This Primary Antibody has been cited in 4+ publications.

Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 was used at a 1:1000 dilution to detect NF2 by Western blot. (Merlin 抗体 (pSer518))

Image no. 2 for anti-Neurofibromin 2 (NF2) (pSer518) antibody (ABIN104130) (Merlin 抗体 (pSer518))

2 images

(4 references)

产品编号 ABIN104130

Supplier Product No.: 600-401-414

发货至: 中国

Quick Overview for Merlin 抗体 (pSer518) (ABIN104130)

抗原

See all Merlin (NF2) 抗体

Merlin (NF2) (Neurofibromin 2 (NF2))

适用

小鼠

宿主

兔

克隆类型

多克隆

标记

This Merlin antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Discover our top product NF2 Primary Antibody

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pSer518

Supplier Product No.

600-401-414

Supplier

Rockland

原理

NF2 phospho S518 Antibody

交叉反应（详细）

This affinity purified antibody is directed against human NF2 (Neurofibromatosis 2 gene product).

产品特性

Synonyms: rabbit anti-NF2 pS518 antibody, rabbit anti-Merlin pS518 antibody, moesin ezrin radixin like protein antibody, Neurofibromatosis 2 antibody, Neurofibromin-2, Schwannomerlin, Schwannomin, Merlin, NF 2, NF-2, SCH

纯化方法

The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

过滤

Sterile filtered

免疫原

Immunogen: Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Conjugated Peptide

亚型

IgG
anti-Neurofibromin 2 (NF2) (AA 477-576) antibody
NF2 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Neurofibromin 2 (NF2) (AA 230-414) antibody
NF2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Neurofibromin 2 (NF2) (AA 30-239) antibody
NF2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Neurofibromin 2 (NF2) (AA 30-311) antibody
NF2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Neurofibromin 2 (NF2) (pSer518) antibody
NF2 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Neurofibromin 2 (NF2) (pSer518) antibody
NF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Neurofibromin 2 (NF2) (AA 1-507) antibody
NF2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Neurofibromin 2 (NF2) (C-Term) antibody
NF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Neurofibromin 2 (NF2) (AA 1-50), (pSer10) antibody
NF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Neurofibromin 2 (NF2) (C-Term) antibody
NF2 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 马, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry Dilution: User Optimized
Application Note: This phospho-specific polyclonal antibody was tested by immunoblotting and ELISA. By ELISA, the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse NF2 (Merlin). Although not tested, this antibody is likely functional in Immunofluorescence, immunohistochemistry, and immunoprecipitation.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:1,200,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Hu, Du, Shelton, Oldham, DiPersio, Klein: "An FAK-YAP-mTOR Signaling Axis Regulates Stem Cell-Based Tissue Renewal in Mice." in: Cell stem cell, Vol. 21, Issue 1, pp. 91-106.e6, (2018) (PubMed).

Angelo, Wu, Meng, Sun, Kopetz, McCutcheon, Slopis, Kurzrock: "Combining Curcumin (Diferuloylmethane) and Heat Shock Protein Inhibition for Neurofibromatosis 2 Treatment: Analysis of Response and Resistance Pathways." in: Molecular cancer therapeutics, (2011) (PubMed).

Lallemand, Saint-Amaux, Giovannini: "Tumor-suppression functions of merlin are independent of its role as an organizer of the actin cytoskeleton in Schwann cells." in: Journal of cell science, Vol. 122, Issue Pt 22, pp. 4141-9, (2010) (PubMed).

Wang, Lu, Tang, Wang, Wu: "The phosphorylation status of merlin in sporadic vestibular Schwannomas." in: Molecular and cellular biochemistry, Vol. 324, Issue 1-2, pp. 201-6, (2009) (PubMed).
抗原

Merlin (NF2) (Neurofibromin 2 (NF2))

别名

NF2

背景

Background: The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin). The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlin's similarity to ERM's suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane. The NF2 protein is highly expressed in human fibroblasts and is detected as a single band of about 75 kDa. This antibody is specific for the phosphorylated form of human NF2 (Merlin).

基因ID

4771

NCBI登录号

NP_000259

UniProt

P35240

途径

Cell-Cell Junction Organization