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MDH1 抗体

This anti-MDH1 antibody is a 绵羊 多克隆 antibody detecting MDH1 in WB, ELISA 和 IP. Suitable for Pig.
Rockland
产品编号 ABIN103904
Supplier Product No.: 200-601-145-0100
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for MDH1 抗体 (ABIN103904)

抗原

See all MDH1 抗体
MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

适用

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Pig

宿主

  • 68
  • 24
  • 3
  • 1
绵羊

克隆类型

  • 60
  • 35
多克隆

标记

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  • 7
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This MDH1 antibody is un-conjugated

应用范围

  • 86
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  • 9
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Western Blotting (WB), ELISA, Immunoprecipitation (IP)
  • Supplier Product No.

    200-601-145-0100

    Supplier

    Rockland

    原理

    Malate Dehydrogenase Antibody

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].

    产品特性

    Synonyms: sheep anti-Malate Dehydrogenase Antibody, Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase

    纯化方法

    Anti-MALATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    免疫原

    Immunogen: Malate Dehydrogenase [Pig Heart]

    Immunogen Type: Native Protein

    亚型

    IgG
  • 应用备注

    Application Note: MALATE DEHYDROGENASE antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Malate Dehydrogenase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度

    1 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    有效期

    12 months
  • 抗原

    MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

    别名

    MDH1

    背景

    Background: Catalytic activity of Malate Dehydrogenase is (S)-malate + NAD+ = oxaloacetate + NADH.

    基因ID

    396894

    UniProt

    P11708
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