MDH1 抗体
Quick Overview for MDH1 抗体 (ABIN103904)
抗原
See all MDH1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-601-145-0100
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Supplier
- Rockland
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原理
- Malate Dehydrogenase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].
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产品特性
- Synonyms: sheep anti-Malate Dehydrogenase Antibody, Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase
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纯化方法
- Anti-MALATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Malate Dehydrogenase [Pig Heart]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) antibody
MDH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B11-B7 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) antibodyMDH1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) antibodyMDH1 适用: 人 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (C-Term) antibodyMDH1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 265-334) antibodyMDH1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) antibodyMDH1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 211-223) antibodyVerified MDH1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 101-193) antibodyMDH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) antibodyMDH1 适用: 人 WB 宿主: 小鼠 Monoclonal CPTC-MDH1-1 unconjugated
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 2-334) antibodyMDH1 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: MALATE DEHYDROGENASE antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Malate Dehydrogenase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
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别名
- MDH1
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背景
- Background: Catalytic activity of Malate Dehydrogenase is (S)-malate + NAD+ = oxaloacetate + NADH.
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基因ID
- 396894
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UniProt
- P11708
抗原
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