Sucrase Isomaltase, Regulatory (SI-R) 抗体 (Biotin)
Our Local Distributor
北京 101111
Quick Overview for Sucrase Isomaltase, Regulatory (SI-R) 抗体 (Biotin) (ABIN103307)
抗原
适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 200-106-189-0100
-
Supplier
- Rockland
-
原理
- Invertase Antibody Biotin Conjugated
-
交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Invertase [Candida species].
-
产品特性
- Synonyms: goat anti-Invertase Antibody biotin Conjugated, Biotin Conjugated goat anti-Invertase Antibody, Beta fructofuranosidase 2 antibody, Inv1 antibody, Saccharase antibody, Sucrase antibody
-
纯化方法
- Anti-Invertase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
-
免疫原
-
Immunogen: Invertase [Candida species]
Immunogen Type: Native Protein
-
亚型
- IgG
-
-
anti-Sucrase Isomaltase, Regulatory (SI-R) antibody
SI-R 适用: Candida sp. ELISA, WB 宿主: 山羊 Polyclonal unconjugated
-
-
应用备注
-
Application Note: Anti-Invertase antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Invertase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
浓度
- 10.0 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- Sucrase Isomaltase, Regulatory (SI-R)
-
别名
- SUC1
-
背景
- Background: Anti-Invertase antibody recognizes invertase which hydrolyzes the alpha-1,4-glycosidic bond between alpha-D-glucose and beta-D-fructose of sucrose. Bees use invertase to convert nectar to honey. In the confectionary industry, yeast-derived invertase is used to hydrolyze sucrose to glucose and fructose as fructose is more sweeter and less prone to crystallization. Anti-Invertase antibody is ideal for investigators interested in Metabolism and Cell Biology.
-
基因ID
- 3640338
-
UniProt
- P10594
抗原
-
