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SLC17A7 抗体 (AA 493-560)

The 小鼠 单克隆 anti-SLC17A7 antibody (Clone S28-9) (ABIN1027710) specifically detects SLC17A7 in WB, IHC 和 mIHC. The antibody is reactive with 大鼠 samples. Independently validated for use in Multiplex Immunohistochemistry.
Independent Validation (1 validation)
产品编号 ABIN1027710
发货至: 中国
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Quick Overview for SLC17A7 抗体 (AA 493-560) (ABIN1027710)

抗原

See all SLC17A7 抗体
SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

适用

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大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This SLC17A7 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Multiplex Immunohistochemistry (mIHC)

克隆位点

S28-9

  • 抗原表位

    • 17
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    AA 493-560

    特异性

    Detects ~52 kDa. No cross-reactivity against VGlut2.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Protein G Purified

    免疫原

    Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

    亚型

    IgG1
  • anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) antibody
    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) antibody

    SLC17A7 适用: 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated

    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 461-560) antibody
    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 461-560) antibody

    SLC17A7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 501-514) antibody
    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 501-514) antibody

    SLC17A7 适用: 人 IHC, IHC (p) 宿主: 山羊 Polyclonal unconjugated

    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (HRP)
    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (HRP)

    SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 HRP

    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (FITC)
    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (FITC)

    SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 FITC

    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (Biotin)
    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (Biotin)

    SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 Biotin

    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (PE)
    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (PE)

    SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 PE

    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (PerCP)
    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (PerCP)

    SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 PerCP

    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (APC)
    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (APC)

    SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 APC

    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (Atto 488)
    anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (Atto 488)

    SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 Atto 488

  • 应用备注

    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.

    说明

    1 μg/ml of ABIN1027710 was sufficient for detection of VGLut1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 生效 #104338 (Multiplex Immunohistochemistry)
    '独立验证'标志
    by
    Akoya Biosciences
    No.
    #104338
    日期
    2021.04.20
    抗原
    SLC17A7
    Lot Number
    1205
    Method validated
    Multiplex Immunohistochemistry
    Positive Control

    Fresh frozen mouse olfactory bulb

    Negative Control

    Unlabeled control (mouse fresh frozen)

    Notes

    Passed. The anti-SLC17A7 antibody ABIN1027710 labels areas of high glutamatergic afferent input throughout the murine olfactory bulb with a concentration in the internal plexiform and granule cell layer.

    '独立验证'标志
    Validation Images
    Murine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-SLC17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550). Labeling is present throughout olfactory bulb layers with a concentration in the internal plexiform and granule cell layers. Map2 and PSD-95/DLG4 were labeled with ABIN125739 (blue; bound to fluorophore ATTO 550) and ABIN361694 (red; bound to fluorophore ATTO 550). Murine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-SLC17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550). Labeling is present throughout olfactory bulb layers with a concentration in the internal plexiform and granule cell layers. Map2 and PSD-95/DLG4 were labeled with ABIN125739 (blue; bound to fluorophore ATTO 550) and ABIN361694 (red; bound to fluorophore ATTO 550).
    FFPE normal human cortex tissue section labeled with anti-SL17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550) after EDTA antigen retrieval. MAP2 and dopamine transporter were labeledd with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-dopamine receptor antibody (blue; bound to fluorophore AF647). FFPE normal human cortex tissue section labeled with anti-SL17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550) after EDTA antigen retrieval. MAP2 and dopamine transporter were labeledd with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-dopamine receptor antibody (blue; bound to fluorophore AF647).
    Full Methods
    Primary Antibody
    ABIN1027710
    Secondary Antibody
    Full Protocol
    • Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
    • Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
    • Conjugation of the anti-SLC17A7 antibody ABIN1027710 to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.
    Experimental Notes

    • No signal was detected in unlabeled specimens.

    • Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

    • Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    -20°C

  • 抗原

    SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

    别名

    VGLUT1

    背景

    VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

    基因ID

    116638

    NCBI登录号

    NP_446311

    UniProt

    Q62634
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