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SLC17A7 抗体 (AA 493-560)

Name: SLC17A7 抗体 (AA 493-560)
Brand: antibodies-online
SKU: ABIN1027710
Availability: OutOfStock
Rating: 5 (1 reviews)

SLC17A7 适用: 大鼠 WB, IHC, mIHC 宿主: 小鼠 Monoclonal S28-9 unconjugated

Multiplex Immunohistochemistry validation image for anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (ABIN1027710) (SLC17A7 抗体 (AA 493-560))

mIHC

Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (ABIN1027710). (SLC17A7 抗体 (AA 493-560))

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (ABIN1027710). (SLC17A7 抗体 (AA 493-560))

ICC

5 images

(1 validation)

产品编号 ABIN1027710

发货至: 中国

Datasheet as PDF

抗原 See all SLC17A7 抗体

SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))
抗原表位
11

5

4

3

1

1

1

1

1

1
AA 493-560
适用
47

34

30

7

6

5

4

2

2

2

1
大鼠
宿主
32

14

2

2

1
小鼠
Compatible Secondaries
克隆类型
37

14
单克隆
标记
22

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This SLC17A7 antibody is un-conjugated
应用范围
47

24

13

7

5

4

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Multiplex Immunohistochemistry (mIHC)

特异性

Detects ~52 kDa. No cross-reactivity against VGlut2.

交叉反应

人, 小鼠, 大鼠

纯化方法

Protein G Purified

免疫原

Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

克隆位点

S28-9

亚型

IgG1
anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) antibody
SLC17A7 适用: 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 501-514) antibody
SLC17A7 适用: 人 IHC, IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 461-560) antibody
SLC17A7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (HRP)
SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 HRP

anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (FITC)
SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 FITC

anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (Alkaline Phosphatase (AP))
SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 Alkaline Phosphatase (AP)

anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (Biotin)
SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 Biotin

anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (PE)
SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 PE

anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (APC)
SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 APC

anti-Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7) (AA 493-560) antibody (PerCP)
SLC17A7 适用: 大鼠 WB, IHC 宿主: 小鼠 Monoclonal S28-9 PerCP

应用备注
- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
说明

1 μg/ml of ABIN1027710 was sufficient for detection of VGLut1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

限制

仅限研究用
生效 #104338 (Multiplex Immunohistochemistry)

by

Akoya Biosciences

No.

#104338

日期

2021.04.20

抗原

SLC17A7

Lot Number

1205

Method validated

Multiplex Immunohistochemistry

Positive Control

Fresh frozen mouse olfactory bulb

Negative Control

Unlabeled control (mouse fresh frozen)

Notes

Passed. The anti-SLC17A7 antibody ABIN1027710 labels areas of high glutamatergic afferent input throughout the murine olfactory bulb with a concentration in the internal plexiform and granule cell layer.

Validation Images

Murine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-SLC17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550). Labeling is present throughout olfactory bulb layers with a concentration in the internal plexiform and granule cell layers. Map2 and PSD-95/DLG4 were labeled with ABIN125739 (blue; bound to fluorophore ATTO 550) and ABIN361694 (red; bound to fluorophore ATTO 550).

FFPE normal human cortex tissue section labeled with anti-SL17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550) after EDTA antigen retrieval. MAP2 and dopamine transporter were labeledd with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-dopamine receptor antibody (blue; bound to fluorophore AF647).
Full Methods
Primary Antibody

ABIN1027710

Secondary Antibody

Full Protocol

Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
Conjugation of the anti-SLC17A7 antibody ABIN1027710 to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.

Experimental Notes

No signal was detected in unlabeled specimens.
Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.
Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

-20°C
抗原

SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

别名

VGLUT1 (SLC17A7 产品)

别名

2900052E22Rik antibody, AI851913 antibody, VGluT1 antibody, Vglut1 antibody, BNPI antibody, VGLUT1 antibody, slc17a7 antibody, vglut1 antibody, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody, solute carrier family 17 member 7 antibody, solute carrier family 17 (vesicular glutamate transporter), member 7a antibody, Slc17a7 antibody, SLC17A7 antibody, slc17a7a antibody

背景

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

基因ID

116638

NCBI登录号

NP_446311

UniProt

Q62634