NIPBL 抗体 (AA 330-365)
Quick Overview for NIPBL 抗体 (AA 330-365) (ABIN100654)
抗原
See all NIPBL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 330-365
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Supplier Product No.
- 600-101-288
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Supplier
- Rockland
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原理
- IDN3 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human IDN3 protein.
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产品特性
- Synonyms: goat anti-IDN3 Antibody, SCC2, NIPBL, CDLS antibody, Colon tumor susceptibility 2 antibody, Delangin antibody, DKFZp434L1319 antibody, FLJ11203 antibody
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 330-365 of Human IDN3.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Nipped-B like Protein (NIPBL) (AA 889-902) antibody
Verified NIPBL 适用: 人 ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Nipped-B like Protein (NIPBL) (AA 2523-2697) antibodyNIPBL 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nipped-B like Protein (NIPBL) (AA 66-175) antibodyNIPBL 适用: 人 ELISA, WB, IF 宿主: 小鼠 Monoclonal 3B9 unconjugated
anti-Nipped-B like Protein (NIPBL) antibodyNIPBL 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect bands at 316 kDa in size corresponding to IDN3 by western blotting in the appropriate cell lysate or extract. Splice variants for this protein may show molecular weights of 304 kDa and 122 kDa.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.6 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- NIPBL (Nipped-B like Protein (NIPBL))
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别名
- IDN3
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背景
- Background: IDN3 (also known as Colon tumor susceptibility 2, DKFZp434L1319, FLJ13648, Mis 4, Scc-2, Delangin and Sister chromatid cohesion protein) is the mammalian homologue of Scc2 in S. cerevisiae and Mis4 in S. pombe. In yeast, this factor was originally identified genetically as being involved in sister chromatid cohesion, subsequent work has determined that it is not involved in cohesion directly but is required for the loading of cohesions onto chromatin during S phase. It shares 37 % sequence identity with Nipped-B gene product of Drosophila, which facilitates enhancer-promoter communication of remote enhancers. The Drosophila protein is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. IDN3 is a nuclear protein that is widely expressed and found at the highest levels in heart, skeletal muscle, fetal and adult liver, and fetal and adult kidney. The protein is also found, albeit at lower levels of expression in thymus, placenta, peripheral leukocyte and small intestine. Multiple isoforms of the protein have been reported.
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基因ID
- 25836, 4760549
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UniProt
- Q6KC79
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途径
- Sensory Perception of Sound, Stem Cell Maintenance
抗原
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