AGO6 抗体
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北京 101111
Quick Overview for AGO6 抗体 (ABIN619533)
抗原
适用
宿主
克隆类型
应用范围
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原理
- Anti-AGO6 | Argonaute 6
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交叉反应 （详细）
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Arabidopsis thaliana
Not reactive in: Poplar sp., Zea mays -
预测反应
- Arabidopsis thaliana
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纯化方法
- Immunogen affinity purified serum
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免疫原
- KLH-conjugated peptide derived from Arabidopsis thaliana AGO6 UniProt: O48771 TAIR:At2g32940
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应用备注
- 1 : 1000 (WB)
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说明
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antibody binds microRNA and tasiRNAs, preference for 24nt siRNAs with 5'UAGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- AGO6 (Argonaute 6 (AGO6))
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别名
- AGO6
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背景
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Background: AGO6 (Argonaute 6) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).
Additional information: There can be a reaction with NEB broad range prestained protein ladder depending upon secondary antibody used.AGO6 accumulates to lower levels in the nrpd1a mutant (Havecker at el. 2010). In addition, a non-specific band migrates to a similar location and thus protein was run on a 6 % denaturing gel to obtain good separation.If other secondary antibody is used than in the figure below, cross-reacting pattern can be different.
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分子量
- 116.4 | 99 kDa
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基因ID
- 817856
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UniProt
- O48771
抗原
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