Caspase-1 / ICE Fluorogenic Substrate 2
产品编号 ABIN924939
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Caspase-1 / ICE Fluorogenic Substrate 2 (ABIN924939)
应用范围
In vitro Assay (in vitro)
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原理
- Peptide substrate labeled at the carboxy end with AFC (7-amino-4-trifluoromethyl coumarin). Designed to measure Caspase-1 or Caspase-4 activity in vitro.
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序列
- Ac-Tyr-Val-Ala-Asp-AFC, Ac-YVAD-AFC
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特异性
- Serves as a substrate for Caspase-1 and as a weak substrate for Caspase-4. Not cleaved by Caspases-2, -3, -6, or -7.
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应用备注
- For in vitro assays of Caspase-1 and Caspase-4 activities. Can be used with purified or partially purified enzymes, or possibly with crude cell lysates (if the Caspase-1/ICE Inhibitor 2 is included to determine background protease activity).
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说明
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Fluorometer calibration: The fluorometer is calibrated using known concentrations of free AFC (Excitation = 400 nm, Emission = 505 nm) to generate a standard curve of fluorescence versus µ-moles AFC. Samples: Can be either purified or partially purified caspase preparations. Application to crude cell lysates has not been confirmed. If crude cell lysates are to be assayed, the non- specific protease background must be determined using our specific Caspase-1/ICE Inhibitor 2.
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限制
- 仅限研究用
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储存条件
- RT
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背景
- Peptide substrate labeled at the carboxy end with AFC (7-amino-4-trifluoromethyl coumarin). Designed to measure Caspase-1 or Caspase-4 activity in vitro. Interleukin-1beta Converting Enzyme (ICE), now termed Caspase-1, is a cytoplasmic cysteine protease that cleaves inactive 31 kDa pro-IL-1beta to generate the active 17.5 kDa proinflammatory cytokine IL-1beta, the predominant form of IL-1 produced by human monocytes. This cytokine has been implicated in the pathogenesis of several diseases such as rheumatoid arthritis, inflammatory bowel disease, and septic shock. Caspase-1/ICE mRNA is found in a variety of cells such as peripheral blood monocytes, peripheral blood lymphocytes, peripheral blood neutrophils, and resting and activated peripheral blood T lymphocytes. The tissue distribution of Caspase-1/ICE suggests that the enzyme may have other substrates in addition to IL-1beta. Current hypotheses suggest that Caspase-1/ICE is able to cause apoptosis as well as activate inflammation in animal cells. Experiments have shown that Caspase-1/ICE has sequence homology with other mammalian apoptosis genes and that activation of Caspase-1/ICE or other ICE-related proteases (caspases)is required for anti-Fas mAb-induced apoptosis.
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分子量
- 720 Da
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