CUT&RUN Concanavalin A Beads
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- 应用范围
- Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Separation (Sep)
- 原理
- CUT&RUN Concanavalin A Beads of our CUT&RUN Sets.
- 特异性
- The unique saccharide-binding properties of plant lectins, such as Concanavalin A have made them useful for the labeling and isolation of glycan-presenting cells and glycoproteins in serum and cell lysate.
- 产品特性
- Concanavalin A is covalently attached to functionalized Magnetic iron oxide particles for use in glycoprotein isolations from serum and cellular extracts.
- 组件
- Concanavalin A coated particles
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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- Metal ions (calcium and manganese) mediate the binding to Concanavalin A and stabilize is conformation.
- The use of buffers with EDTA or other metal chelators must be avoided as it will result in a loss of carbohydrate binding ability.
- 试剂准备
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Prepare CUT&RUN Concanavalin A Beads
- Pipette 10 µL CUT&RUN Concanavalin A Beads slurry for each sample into a tube
- Place the tubes on a magnet separator and remove the liquid carefully
- Remove the tubes from the magnetic separator
- Wash beads 3 more times with 1 mL Binding Buffer
- Finally resuspend the beads with 10 µL Binding Buffer per sample
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 5 μg/μL
- 缓冲液
- Stored in 10 mM PBS with 0.1 % Sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze the product!
Vortex bead suspension well before use. - 储存条件
- 4 °C
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