CUT&RUN Concanavalin A Beads
Quick Overview for CUT&RUN Concanavalin A Beads (ABIN6923139)
应用范围
Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag), Separation (Sep)
-
-
原理
- CUT&RUN Concanavalin A Beads of our CUT&RUN Sets.
-
特异性
- The unique saccharide-binding properties of plant lectins, such as Concanavalin A have made them useful for the labeling and isolation of glycan-presenting cells and glycoproteins in serum and cell lysate.
-
产品特性
- Concanavalin A is covalently attached to functionalized Magnetic iron oxide particles for use in glycoprotein isolations from serum and cellular extracts.
-
组件
- Concanavalin A coated particles
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
- Metal ions (calcium and manganese) mediate the binding to Concanavalin A and stabilize is conformation.
- The use of buffers with EDTA or other metal chelators must be avoided as it will result in a loss of carbohydrate binding ability.
-
试剂准备
-
Prepare CUT&RUN Concanavalin A Beads
- Pipette 10 µL CUT&RUN Concanavalin A Beads slurry for each sample into a tube
- Place the tubes on a magnet separator and remove the liquid carefully
- Remove the tubes from the magnetic separator
- Wash beads 3 more times with 1 mL Binding Buffer
- Finally resuspend the beads with 10 µL Binding Buffer per sample
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 5 μg/μL
-
缓冲液
- Stored in 10 mM PBS with 0.1 % Sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
-
Do not freeze the product!
Vortex bead suspension well before use. -
储存条件
- 4 °C
-
-
You are here:
