Anti-DYKDDDDK G1 Affinity Resin
Quick Overview for Anti-DYKDDDDK G1 Affinity Resin (ABIN2017617)
抗原
DYKDDDDK Tag
应用范围
Immunoprecipitation (IP), Purification (Purif), Separation (Sep)
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适用
- 标记
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克隆类型
- 单克隆
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特异性
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High DYKDDDDK-tag binding capacity and ultralow levels of non-specific binding
Multiple elution methods for diverse protein characteristics and different requirements
Used for purification and immunoprecipitation of DYKDDDDK-tagged protein or protein complex -
产品特性
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DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Anti-DYKDDDDK G1 Affinity Resin is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK-tagged protein in the cell lysate can specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate.
Matrix: 4 % cross-linked agarose
Average bead size: 90 μm
Ligand: Mouse monoclonal antibody against DYKDDDDK tag, clone G1
Binding capacity: Approximately 1 mg DYKDDDDK-tagged protein per ml settled resin
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说明
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Resin reuse: The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.
Elution method: Acid, alkaline, neutral, peptide competitive and SDS-PAGE loading buffer elutions
Reagents compatibility: Compatible with commonly used reagents at certain concentrations -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 50 % settled resin in TBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze the resin.
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C for up to 12 months.
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有效期
- 12 months
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- DYKDDDDK Tag
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别名
- DYKDDDDK
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物质类
- Tag
抗原
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