(1 reference)Quick Overview for Phospho ERK1/2 Flex Set (Bead C4) (ABIN1379692)
抗原
ERK1/2 (MAPK1/3)
(Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
应用范围
Immunoassay (IA), Flow Cytometry (FACS)
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抗原表位
- pThr202, pTyr204
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适用
- 人, 小鼠, 大鼠
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品牌
- BD™ Cytometric Bead Array,BD™
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产品特性
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The BD™ CBA Phospho ERK1/2 (T202/Y204) Flex Set is a bead-based immunoassay capable of measuring human, mouse, or rat extracellular signal regulated kinases 1/2 (ERK1/2), a member of the mitogen-activated protein kinase (MAPK) family of kinases, that has been threonine-phosphorylated at Thr-202 and tyrosine-phosphorylated at Tyr-204 in denatured cell lysate samples. Human and mouse reactivity was determined by testing cell lysates with the BD CBA Phospho ERK1/2 (T202/Y204) Flex Set. Reactivity with rat samples was determined by western blot for each of the antibodies used in the BD CBA Phospho ERK1/2 (T202/Y204) Flex Set. The biology and function of ERK1/2 has been previously reviewed. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005 or 560006).
This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light.
The Phospho ERK1/2 (T202/Y204) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use. -
组件
- Capture beads, detection reagent, standards
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说明
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Bead Position: C4
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限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
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缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- This BD™ CBA Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light. The Phospho ERK1/2 (T202/Y204) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the BD CBA Cell Signaling Master Buffer Kit (Cat. No. 560005/560006), warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.
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: "Targeting MAPK signalling: Prometheus' fire or Pandora's box?" in: Current pharmaceutical design, Vol. 10, Issue 16, pp. 1885-905, (2004) (PubMed).
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: "Targeting MAPK signalling: Prometheus' fire or Pandora's box?" in: Current pharmaceutical design, Vol. 10, Issue 16, pp. 1885-905, (2004) (PubMed).
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- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
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别名
- ERK1/2
抗原
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