人 IgG3 lambda G3m (g)
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- 适用
- 人
- 宿主
- Human
- 应用范围
- Haemagglutination (H), Immunodiffusion (ID), Immunoelectrophoresis (IEP), ELISA, Western Blotting (WB)
- 产品特性
- Confirmed by immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). Not less than 98% as deter- mined by agar-gel electrophoresis, quantitative densitometry immunoelectrophoresis and double radial immunodiffusion (Ouchterlony).
- 纯化方法
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Non-antisera
The protein is isolated from plasma using ion exchange chromatography, affinity chromatography and gelfiltration.
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- 说明
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Physical form: Lyophilized, stable white powder.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 储存液
- Without preservative
- 注意事项
- This product is shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. The lyophilized content is reconstituted by adding 0.5 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
- 储存条件
- 4 °C
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- 背景
- Purified human IgG3 lambda may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelectrophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
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